Evidence map›Paper›PMID 31723027›Full record

ArticleJournal of virology2020

Genome-Wide Mutagenesis of Hepatitis C Virus Reveals Ability of Genome To Overcome Detrimental Mutations.

Deepak Singh, Shalini Soni, Shaheen Khan, Aditya N Sarangi, Ragothaman M Yennamalli, Rakesh Aggarwal, Naga Suresh Veerapu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. HCV Replicon Systems: Workhorses of Drug Discovery and Resistance.Frontiers in cellular and infection microbiology · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Deepak Singh *Virology Section, Department of Life Sciences, Shiv Nadar University, Gautam Buddha Nagar, Uttar Pradesh, India.
Shalini Soni *Virology Section, Department of Life Sciences, Shiv Nadar University, Gautam Buddha Nagar, Uttar Pradesh, India.
Shaheen KhanVirology Section, Department of Life Sciences, Shiv Nadar University, Gautam Buddha Nagar, Uttar Pradesh, India.
Aditya N SarangiGastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
Ragothaman M YennamalliTranslational Genomics and Immunomics Unit, VivagenDx Labs, Chennai, Tamil Nadu, India.
Rakesh AggarwalGastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
Naga Suresh VeerapuVirology Section, Department of Life Sciences, Shiv Nadar University, Gautam Buddha Nagar, Uttar Pradesh, India nagasuresh.veerapu@snu.edu.in.
Shiv Nadar University · INSanjay Gandhi Post Graduate Institute of Medical Sciences · INSciGenom Labs (India) · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To gain insight into the impact of mutations on the viability of the hepatitis C virus (HCV) genome, we created a set of full-genome mutant libraries, differing from the parent sequence as well as each other, by using a random mutagenesis approach; the proportion of mutations increased across these libraries with declining template amount or dATP concentration. The replication efficiencies of full-genome mutant libraries ranged between 71 and 329 focus-forming units (FFU) per 10

Indexed as

Genome, ViralMutagenesisMutationCell LineDEAD-box RNA HelicasesHepacivirusHumansModels, MolecularNucleoside-TriphosphatasePhenotypeSerial PassageSerine EndopeptidasesViral Nonstructural ProteinsViral ProteasesViral ProteinsVirus ReplicationDEAD-box RNA HelicasesNS3 protein, hepatitis C virusNucleoside-TriphosphataseSerine EndopeptidasesViral Nonstructural ProteinsViral ProteasesViral Proteinsgenome viabilitygenome-wide mutagenesishepatitis C virusquasispeciesreverse genetic analysistolerance

Identifiers

PMID31723027
PMCPMC7000968
OpenAlexW2987692152

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.