Evidence map›Paper›PMID 31722603›Full record

ArticleSLAS technology2020

Distance and Microsphere Aggregation-Based DNA Detection in a Paper-Based Microfluidic Device.

Brent Kalish, Jianhou Zhang, Hilary Edema, James Luong, Jenna Roper, Chad Beaudette, Richard Echodu, Hideaki Tsutsui

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in SLAS technology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Brent KalishDepartment of Mechanical Engineering, University of California Riverside, Riverside, CA, USA.
Jianhou ZhangDepartment of Mechanical Engineering, University of California Riverside, Riverside, CA, USA.
Hilary EdemaGulu University Bioscience Research Laboratories, Gulu, Uganda.
James LuongDepartment of Mechanical Engineering, University of California Riverside, Riverside, CA, USA.
Jenna RoperDepartment of Bioengineering, University of California Riverside, Riverside, CA, USA.
Chad BeaudetteDepartment of Mechanical Engineering, University of California Riverside, Riverside, CA, USA.
Richard EchoduGulu University Bioscience Research Laboratories, Gulu, Uganda.
Hideaki TsutsuiDepartment of Mechanical Engineering, University of California Riverside, Riverside, CA, USA.ORCID 0000-0002-2558-2639

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In paper-based microfluidics, the simplest devices are colorimetric, giving qualitative results. However, getting quantitative data can be quite a bit more difficult. Distance-based devices provide a user-friendly means of obtaining quantitative data without the need for any additional equipment, simply by using an included ruler or calibrated markings. This article details the development of a quantitative DNA detection device that utilizes the aggregation of polystyrene microspheres to affect the distance that microspheres wick through filter paper. The microspheres are conjugated to single-stranded DNA (ssDNA) oligomers that are partially complementary to a target strand and, in the presence of the target strand, form a three-strand complex, resulting in the formation of aggregates. The higher the concentration of the target strand, the larger the aggregate, and the shorter the distance wicked by the microspheres. This behavior was investigated across a wide range of target concentrations and under different incubation times to understand aggregate formation. The device was then used to successfully detect a target strand spiked in extracted plant DNA.

Indexed as

Capillary ActionDNAMicrofluidic Analytical TechniquesMicrospheresPaperPlant ExtractsDNAPlant Extractsaggregationdistance-basedDNA detectionmicrospherepaper-based

Identifiers

PMID31722603
PMCPMC6974776

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.