ArticlePloS one2019
16S rDNA droplet digital PCR for monitoring bacterial DNAemia in bloodstream infections.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Arabidopsis DNA repair mutants can integrate Agrobacterium T-DNA into the plant genome.The New phytologist · 2026Article
- Diagnostic advantage of droplet digital PCR over blood culture in sepsis: a retrospective study of 115 patients.European journal of medical research · 2026Article
- Metagenomics next-generation sequencing of plasma combined with blood cells for improving the prognosis of early infection in patients with hematologic disorders: a real-world cohort study in northern China.Frontiers in molecular biosciences · 2026Article
- Development of a multiplex ddPCR assay for simultaneous absolute quantification of bacterial, fungal, and human DNA.PloS one · 2026Article
- RNA profiles in extracellular vesicles from severe sepsis and meningitis patients reveal pathogen-specific immune signatures in meningococcal versus pneumococcal infections.Frontiers in cellular and infection microbiology · 2026Article
- Letter to the editor regarding "microbiologic analysis of 140 patients undergoing revision total knee arthroplasty due to periprosthetic joint infection and aseptic loosening: droplet digital PCR analysis".Journal of orthopaedic surgery and research · 2025Article
- Absolute quantification of prokaryotes in the microbiome by 16S rRNA qPCR or ddPCR.Nature protocols · 2025Review
- Quantification of Bacterial and Drug-Resistant DNA Using dPCR in a Pediatric Patient with CVC-Related Bloodstream Infection.Infectious disease reports · 2025Article
- A suite of ddPCR assays targeting microbial pathogens for improved management of shellfish aquaculture.Applied and environmental microbiology · 2025Article
- Performance of ddPCR-GNB for microbial diagnosis of suspected bloodstream infection due to the four most common gram-negative bacteria: a prospective, multicenter study.Microbiology spectrum · 2025Article
- Clinical diagnostic performance of droplet digital PCR for pathogen detection in patients with Escherichia coli bloodstream infection: a prospective observational study.BMC infectious diseases · 2025Observational
- Critical Steps in Shotgun Metagenomics-Based Diagnosis of Bloodstream Infections Using Nanopore Sequencing.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2025Article
- ddPCR Enhances early diagnosis, treatment, prognosis, and pathogen verification in elderly BSI.Frontiers in cellular and infection microbiology · 2025Article
- Damage-associated molecular patterns in bacteraemic infection, including a comparative analysis with bacterial DNA, a pathogen-associated molecular pattern.Scientific reports · 2024Article
- A retrospective study of the detection of sepsis pathogens comparing blood culture and culture-independent digital PCR.Heliyon · 2024Article
- A new multiplexed magnetic capture-Droplet digital PCR tool for monitoring wildlife population health and pathogen surveillance.Ecology and evolution · 2023Article
- Mucositis and Infection in Hematology Patients.International journal of molecular sciences · 2023Article
- Pathogen load and species monitored by droplet digital PCR in patients with bloodstream infections: A prospective case series study.BMC infectious diseases · 2022Article
- Digital PCR applications for the diagnosis and management of infection in critical care medicine.Critical care (London, England) · 2022Review
- ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples.BMC microbiology · 2021Article
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Authors and funding
5 authors.
Funding
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Abstract
Repeated quantitative measurement of bacterial DNA on whole blood has been shown to be a promising method for monitoring bloodstream infection (BSI) with selected bacterial species. To enable broad use of this method, we developed a quantitative droplet digital PCR (ddPCR) method for 16S rDNA. It was validated with species-specific ddPCRs for Staphylococcus aureus (nuc), Streptococcus pneumoniae (lytA), and Escherichia coli (uidA) on spiked whole blood samples and on repeated whole blood samples (days 0, 1-2, 3-4, 6-8, and 13-15) from 83 patients with BSI with these pathogens. In these patients, 16S rDNA and species-specific DNA were detected in 60% and 61%, respectively, at least at one time-point. The highest positivity rates were seen in S. aureus BSI, where 92% of the patients were 16S rDNA-positive and 85% nuc-positive. Quantitative 16S rDNA and species-specific DNA showed strong correlations in spiked samples (r = 0.98; p < 0.0001) and clinical samples (r = 0.84; p < 0.0001). Positivity for 16S rDNA was rapidly cleared in patients with S. pneumoniae and E. coli BSI, but more slowly and sometimes persisted, in those with S. aureus BSI. The initial 16S rDNA load was higher in BSI patients with sepsis (Sepsis-3 definition) than without sepsis (median 2.38 vs. 0 lg10 copies/mL; p = 0.031) and in non-survivors than in survivors (median 2.83 vs. 0 lg10 copies/mL; p = 0.006). 16S rDNA ddPCR appears to be a promising method for bacterial DNA monitoring during BSI. The clinical value of such monitoring should be further studied.
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