Evidence map›Paper›PMID 31721541›Full record

ArticleJournal of biomedical optics2019

Three-dimensional fluorescence imaging using the transport of intensity equation.

Sudheesh K Rajput, Manoj Kumar, Xiangyu Quan, Mitsuhiro Morita, Tomoyuki Furuyashiki, Yasuhiro Awatsuji, Enrique Tajahuerce, Osamu Matoba

Open access · goldAbstract read
In one paragraph

Article in Journal of biomedical optics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
3.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 41 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Sudheesh K RajputKobe University, Graduate School of System Informatics, Department of Systems Science, Nada, Kobe, Japan.
Manoj KumarKobe University, Graduate School of System Informatics, Department of Systems Science, Nada, Kobe, Japan.
Xiangyu QuanKobe University, Graduate School of System Informatics, Department of Systems Science, Nada, Kobe, Japan.
Mitsuhiro MoritaKobe University, Graduate School of Sciences, Department of Biology, Nada, Kobe, Japan.
Tomoyuki FuruyashikiKobe University, Graduate School of Medicine, Division of Pharmacology, Chuo-ku, Kobe, Japan.
Yasuhiro AwatsujiKyoto Institute of Technology, Faculty of Electrical Engineering and Electronics, Matsugasaki, Sakyo, Japan.
Enrique TajahuerceUniversitat Jaume I, Institute of New Imaging Technologies (INIT), Department of Physics, Castello, Spain.
Osamu MatobaKobe University, Graduate School of System Informatics, Department of Systems Science, Nada, Kobe, Japan.
Kobe University · JPKyoto Institute of Technology · JPUniversitat Jaume I · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We propose a nonscanning three-dimensional (3-D) fluorescence imaging technique using the transport of intensity equation (TIE) and free-space Fresnel propagation. In this imaging technique, a phase distribution corresponding to defocused fluorescence images with a point-light-source-like shape is retrieved by a TIE-based phase retrieval algorithm. From the obtained phase distribution, and its corresponding amplitude distribution, of the defocused fluorescence image, various images at different distances can be reconstructed at the desired plane after Fresnel propagation of the complex wave function. Through the proposed imaging approach, the 3-D fluorescence imaging can be performed in multiple planes. The fluorescence intensity images are captured with the help of an electrically tunable lens; hence, the imaging technique is free from motion artifacts. We present experimental results corresponding to microbeads and a biological sample to demonstrate the proposed 3-D fluorescence imaging technique.

Indexed as

FluorescenceImaging, Three-DimensionalAlgorithmsArtifactsComputer SimulationImage Processing, Computer-AssistedMathematics3-D fluorescence imagingFresnel propagationphase retrievaltransport of intensity equation

Identifiers

PMID31721541
PMCPMC7010985
OpenAlexW2983943133

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.