ArticleCell journal2020
Optimizing The Cell Seeding Protocol to Human Decellularized Ovarian Scaffold: Application of Dynamic System for Bio-Engineering.
Article in Cell journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- Bioengineered ovary reconstructs follicle-like structures in a mouse model of chemotherapy-induced premature ovarian failure.Stem cell research & therapy · 2025Article
- Gintonin-EnrichedFoods (Basel, Switzerland) · 2025Article
- Comparison between wharton's jelly and collagen natural hydrogels for human ovarian follicle transplantation as an artificial ovary.PloS one · 2025Article
- In-vivo oogenesis of oogonial and mesenchymal stem cells seeded in transplanted ovarian extracellular matrix.Journal of ovarian research · 2023Article
- Advanced bioengineering of female germ cells to preserve fertility.Biology of reproduction · 2022Review
- Construction of Artificial Ovaries with Decellularized Porcine Scaffold and Its Elicited Immune Response after Xenotransplantation in Mice.Journal of functional biomaterials · 2022Article
- Two-Decade Experience of Royan Institute in Obtaining Mature Oocyte from Cryopreserved Ovarian Tissue: In Vitro and In Vivo Approaches.Reproductive sciences (Thousand Oaks, Calif.) · 2022Review
- Polymeric Hydrogels for In Vitro 3D Ovarian Cancer Modeling.International journal of molecular sciences · 2022Review
- Characteristics of a decellularized human ovarian tissue created by combined protocols and its interaction with human endometrial mesenchymal cells.Progress in biomaterials · 2021Article
- Recent Advancements in Engineered Biomaterials for the Regeneration of Female Reproductive Organs.Reproductive sciences (Thousand Oaks, Calif.) · 2021Review
- Towards a bioengineered uterus: bioactive sheep uterus scaffolds are effectively recellularized by enzymatic preconditioning.NPJ Regenerative medicine · 2021Article
- Decellularization and recellularization of the ovary for bioengineering applications; studies in the mouse.Reproductive biology and endocrinology : RB&E · 2020Article
- Engineered reproductive tissues.Nature biomedical engineering · 2020Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveDecellularized tissue scaffolds provide an extracellular matrix to control stem cells differentiation toward specific lineages. The application of mesenchymal stem cells for artificial ovary production may enhance MATERIALS AND
methodsIn this experimental study, three methods were used for cellular seeding including rotational (spinner flask) and static (conventional and injection) seeding cultures. OVECs were evaluated with Hematoxylin and Eosin staining and viability analyses for the seeded PMSCs. Then, immunohistochemistry analysis was performed using the best method of cellular seeding for primordial germ cell-like cells, mesenchymal stem cells and proliferation markers. Stereology analysis was also performed for the number of penetrated cells into the OVECs.
resultOur results showed that rotational seeding increases the permeability of PMSCs into the scaffold and survival rate of the seeded PMSCs, comparing to the other methods. On the other hand, rotationally seeded PMSCs had a more favorable capability of proliferation with Ki67 expression and differentiation to ovarian specific cells with expression of primordial germ cell line markers without mesenchymal stem cells markers production. Furthermore, stereology showed a more favorable distribution of PMSCs along the outer surfaces of the OVEC with further distribution at the central part of the scaffold. The average total cell values were determined 2142187 cells/mm
conclusionThe rotational seeding method is a more favorable approach to cell seeding into ovarian decellularized tissue than static seeding.
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