ArticleCell journal2020
Investigation of VASA Gene and Protein Expression in Neonate and Adult Testicular Germ Cells in Mice In Vivo and In Vitro.
Article in Cell journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 17 citations in OpenAlex.
- Low-level lasers can improve spermatogenesis in infertile mice by targeting molecular pathways.BioImpacts : BI · 2026Article
- The dynamics of ciliogenesis in prepubertal mouse meiosis reveals new clues about testis maturation.Frontiers in cell and developmental biology · 2026Article
- Article
- Sox9 downregulation in non-obstructive azoospermia by UTF1 and mediator role of POU5F1.BMC research notes · 2024Article
- Article
- Meiotic Cas9 expression mediates gene conversion in the male and female mouse germline.PLoS biology · 2021Article
- Successful transplantation of spermatogonial stem cells into the seminiferous tubules of busulfan-treated mice.Reproductive health · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveWe aimed to examine the expression levels of the VASA gene and protein in testis sections of neonate and adult mice as well as testicular cell cultures. MATERIALS AND
methodsIn this experimental study, in order to investigate the expression of this germ cell marker gene in more detail, we analyzed the expression of VASA by immunocytochemistry, immunohistochemistry and fluidigm reverse transcription-polymerase chain reaction (RT-PCR).
resultsThe immunohistochemical assays showed that the VASA protein was exclusively expressed in germ cells in the seminiferous tubules of the neonate and adult testis and not in somatic cells. VASA was not detectable in PLZF positive spermatogonial stem cells (SSCs), was weakly expressed in proliferating spermatogonia, and became abundant in spermatocytes and round spermatozoa. Counting VASA-positive cells in the seminiferous tubules of the neonate and adult testis depicted significant higher expression (P<0.05) of VASA in the adult testis in comparison to its neonate counterpart. SSC colonies were established
conclusionThe VASA protein is, therefore, an extremely specific marker of testicular germ cell differentiation
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