Evidence map›Paper›PMID 31719923›Full record

ArticleCellular and molecular bioengineering2019

Injectable, Hyaluronic Acid-Based Scaffolds with Macroporous Architecture for Gene Delivery.

Arshia Ehsanipour, Tommy Nguyen, Tasha Aboufadel, Mayilone Sathialingam, Phillip Cox, Weikun Xiao, Christopher M Walthers, Stephanie K Seidlits

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular bioengineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 31 citations in OpenAlex.

  1. Article
  2. The Evolution of Technology-Driven In Vitro Models for Neurodegenerative Diseases.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Review
  3. Review
  4. Article
  5. Injectable antibacterial Ag-HA/ GelMA hydrogel for bone tissue engineering.Frontiers in bioengineering and biotechnology · 2023
    Article
  6. Article
  7. Review
  8. Review
  9. Nucleic Acid Delivery from Granular Hydrogels.Advanced healthcare materials · 2022
    Article
  10. Review
  11. Review
  12. Review
  13. Review
  14. Biopolymer-based Carriers for DNA Vaccine Design.Angewandte Chemie (International ed. in English) · 2021
    Review
  15. Review
  16. Review
  17. Article
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Arshia EhsanipourDepartment of Bioengineering, University of California Los Angeles, Los Angeles, CA 90095 USA.
Tommy NguyenDepartment of Bioengineering, University of California Los Angeles, Los Angeles, CA 90095 USA.
Tasha AboufadelDepartment of Bioengineering, University of California Los Angeles, Los Angeles, CA 90095 USA.
Mayilone SathialingamDepartment of Bioengineering, University of California Los Angeles, Los Angeles, CA 90095 USA.
Phillip CoxDepartment of Bioengineering, University of California Los Angeles, Los Angeles, CA 90095 USA.
Weikun XiaoDepartment of Bioengineering, University of California Los Angeles, Los Angeles, CA 90095 USA.
Christopher M WalthersDepartment of Bioengineering, University of California Los Angeles, Los Angeles, CA 90095 USA.
Stephanie K SeidlitsDepartment of Bioengineering, University of California Los Angeles, Los Angeles, CA 90095 USA.ORCID 0000-0003-3881-7166
University of California, Los Angeles · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionBiomaterials can provide localized reservoirs for controlled release of therapeutic biomolecules and drugs for applications in tissue engineering and regenerative medicine. As carriers of gene-based therapies, biomaterial scaffolds can improve efficiency and delivery-site localization of transgene expression. Controlled delivery of gene therapy vectors from scaffolds requires cell-scale macropores to facilitate rapid host cell infiltration. Recently, advanced methods have been developed to form injectable scaffolds containing cell-scale macropores. However, relative efficacy of

methodsThree types of scaffolds-nanoporous HA hydrogels (NP-HA), annealed HA microparticles (HA-MP) and nanoporous HA hydrogels containing protease-degradable poly(ethylene glycol) (PEG) microparticles as sacrificial porogens (PEG-MP)-were loaded with lentiviral particles encoding reporter transgenes and injected into mouse mammary fat. Scaffolds were evaluated for their ability to induce rapid infiltration of host cells and subsequent transgene expression.

resultsCell densities in scaffolds, distances into which cells penetrated scaffolds, and transgene expression levels significantly increased with delivery from HA-MP, compared to NP-HA and PEG-MP, scaffolds. Nearly 8-fold greater cell densities and up to 16-fold greater transgene expression levels were found in HA-MP, over NP-HA, scaffolds. Cell profiling revealed that within HA-MP scaffolds, macrophages (F4/80+), fibroblasts (ERTR7+) and endothelial cells (CD31+) were each present and expressed delivered transgene.

conclusionsResults demonstrate that injectable scaffolds containing cell-scale macropores in an open, interconnected architecture support rapid host cell infiltration to improve efficiency of biomaterial-mediated gene delivery.

Indexed as

Gene therapyInjectable scaffoldLentivirusTissue engineering

Identifiers

PMID31719923
PMCPMC6816628
OpenAlexW2972305571

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.