ArticleCellular and molecular bioengineering2019
Characterization of 3D Printed Stretching Devices for Imaging Force Transmission in Live-Cells.
Article in Cellular and molecular bioengineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- A Computational Model of Mechanical Stretching of Cultured Cells on a Flexible Membrane.Research square · 2026Article
- The effects of mechanical stimulation on the morphology and function of spinal ligament cells.BMC musculoskeletal disorders · 2025Article
- Nucleo-cytoskeletal coupling controls intracellular deformation partitioning during cell stretching.Royal Society open science · 2025Article
- Local soft niches in mechanically heterogeneous primary tumors promote brain metastasis via mechanotransduction-mediated HDAC3 activity.Science advances · 2025Article
- Cell tumbling enhances stem cell differentiation in hydrogels via nuclear mechanotransduction.Nature materials · 2025Article
- The cytoskeleton dynamics-dependent LINC complex in periodontal ligament stem cells transmits mechanical stress to the nuclear envelope and promotes YAP nuclear translocation.Stem cell research & therapy · 2024Article
- The VE-cadherin/AmotL2 mechanosensory pathway suppresses aortic inflammation and the formation of abdominal aortic aneurysms.Nature cardiovascular research · 2023Article
- Histone methyltransferase SUV39H1 regulates the Golgi complex via the nuclear envelope-spanning LINC complex.PloS one · 2023Article
- The CaT stretcher: An open-source system for delivering uniaxial strain to cells and tissues (CaT).Frontiers in bioengineering and biotechnology · 2022Article
- A Loss of Nuclear-Cytoskeletal Interactions in Vascular Smooth Muscle Cell Differentiation Induced by a Micro-Grooved Collagen Substrate Enabling the Modeling of an In Vivo Cell Arrangement.Bioengineering (Basel, Switzerland) · 2021Article
- The LINC complex is required for endothelial cell adhesion and adaptation to shear stress and cyclic stretch.Molecular biology of the cell · 2021Article
- Spatial distribution of lamin A/C determines nuclear stiffness and stress-mediated deformation.Journal of cell science · 2021Article
- Design of a 3D printed, motorized, uniaxial cell stretcher for microscopic and biochemical analysis of mechanotransduction.Biology open · 2021Article
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Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
introductionCell stretch is a method which can rapidly apply mechanical force through cell-matrix and cell-cell adhesions and can be utilized to better understand underlying biophysical questions related to intracellular force transmission and mechanotransduction.
methods3D printable stretching devices suitable for live-cell fluorescent imaging were designed using finite element modeling and validated experimentally. These devices were then used along with FRET based nesprin-2G force sensitive biosensors as well as live cell fluorescent staining to understand how the nucleus responds to externally applied mechanical force in cells with both intact LINC (linker of nucleoskeleton and cytoskeleton) complex and cells with the LINC complex disrupted using expression of dominant negative KASH protein.
resultsThe devices were shown to provide a larger strain ranges (300% uniaxial and 60% biaxial) than currently available commercial or academic designs we are aware of. Under uniaxial deformation, the deformation of the nucleus of NIH 3T3 cells per unit of imposed cell strain was shown to be approximately 50% higher in control cells compared to cells with a disrupted LINC complex. Under biaxial deformation, MDCK II cells showed permanent changes in the nuclear morphology as well as actin organization upon unloading, indicating that failure, plastic deformation, or remodeling of the cytoskeleton is occurring in response to the applied stretch.
conclusionDevelopment and open distribution of low-cost, 3D-printable uniaxial and biaxial cell stretching devices compatible with live-cell fluorescent imaging allows a wider range of researchers to investigate mechanical influences on biological questions with only a minimal investment of resources.
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