ArticleCellular and molecular bioengineering2019
Mechanical Characterization of 3D Ovarian Cancer Nodules Using Brillouin Confocal Microscopy.
Article in Cellular and molecular bioengineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- TheMaterials today. Bio · 2026Review
- Macrophages Mediate Mesoscale Brain Mechanical Homeostasis.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Exploring the biomechanical complexity of glioblastoma spheroids and organoids with co-localized Brillouin and Raman microspectroscopy.Biochemistry and biophysics reports · 2025Article
- Cellular mechanical properties in response to environmental viscosity imaged by Brillouin Microscopy.Communications biology · 2025Article
- Unlocking the therapeutic potential of cellular mechanobiology.Science advances · 2025Review
- Imaging the mechanical properties of a developing embryonic tendon using Brillouin microscopy.Acta biomaterialia · 2025Article
- Targeting cancer cell stiffness and metastasis with clinical therapeutics.Clinical & experimental metastasis · 2025Review
- Cell spheroid micromechanics under large deformations.Scientific reports · 2025Article
- Mechanical Evolution of Metastatic Cancer Cells in 3D Microenvironment.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Remodeling of extracellular matrix collagen IV by MIG-6/papilin regulates neuronal architecture.Research square · 2025Article
- Multifaceted cancer alleviation by cowpea mosaic virus in a bioprinted ovarian cancer peritoneal spheroid model.Biomaterials · 2024Article
- Harnessing quantum light for microscopic biomechanical imaging of cells and tissues.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Mechanical evolution of metastatic cancer cells in three-dimensional microenvironment.bioRxiv : the preprint server for biology · 2024Article
- Evidence and therapeutic implications of biomechanically regulated immunosurveillance in cancer and other diseases.Nature nanotechnology · 2024Review
- Network Viscoelasticity from Brillouin Spectroscopy.Biomacromolecules · 2024Article
- Analyzing force measurements of multi-cellular clusters comprising indeterminate geometries.Biomechanics and modeling in mechanobiology · 2024Article
- Brillouin microscopy.Nature reviews. Methods primers · 2024Article
- A Novel Role of Connective Tissue Growth Factor in the Regulation of the Epithelial Phenotype.Cancers · 2023Article
- Classification of cancer cells at the sub-cellular level by phonon microscopy using deep learning.Scientific reports · 2023Article
- Correlation of biomechanics and cancer cell phenotype by combined Brillouin and Raman spectroscopy of U87-MG glioblastoma cells.Journal of the Royal Society, Interface · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
introductionThe mechanical interaction between cells and their microenvironment is emerging as an important determinant of cancer progression and sensitivity to treatment, including in ovarian cancer (OvCa). However, current technologies limit mechanical analysis in 3D culture systems. Brillouin Confocal Microscopy is an optical non-contact method to assess the mechanical properties of biological materials. Here, we validate the ability of this technology to assess the mechanical properties of 3D tumor nodules.
methodsOvCa cells were cultured in 3D using two established methods: (1) overlay cultures on Matrigel; (2) spheroids in ultra-low attachment plates. To alter the mechanical state of these tumors, nodules were immersed in PBS with varying levels of sucrose to induce osmotic stress. Next, nodule mechanical properties were measured by Brillouin microscopy and validated with standard stress-strain tests: Atomic Force Microscopy (AFM) and a parallel plate compression device (Microsquisher). Finally, the nodules were treated with a chemotherapeutic commonly used to manage OvCa, carboplatin, to determine treatment-induced effects on tumor mechanical properties.
resultsBrillouin microscopy allows mechanical analysis with limited penetration depth (~ 92 µm for Matrigel method; ~ 54 µm for low attachment method). Brillouin microscopy metrics displayed the same trends as the corresponding "gold-standard" Young's moduli measured with stress-strain methods when the osmolality of the medium was increased. Nodules treated with carboplatin showed a decrease in Brillouin frequency shift.
conclusionThis validation study paves the way to evaluate the mechanics of 3D nodules, with micron-scale three-dimensional resolution and without contact, thus extending the experimental possibilities.
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