Evidence map›Paper›PMID 31718599›Full record

ArticleBMC urology2019

Langerhans cells in hypospadias: an analysis of Langerin (CD207) and HLA-DR on epidermal sheets and full thickness skin sections.

Bernhard Haid, Daniela Reider, Felix Nägele, Anne-Françoise Spinoit, Elisabeth Pechriggl, Nikolaus Romani, Helga Fritsch, Josef Oswald

Open access · goldAbstract readMulticenter Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC urology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Protective Barriers Provided by the Epidermis.International journal of molecular sciences · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 4 countries.

Bernhard HaidDepartment of Pediatric Urology, Hospital of the Sisters of Charity, Ordensklinikum Linz, Seilerstätte 4, 4020, Linz, Austria. bernhard.haid@ordensklinikum.at.ORCID http://orcid.org/0000-0003-1691-7510
Daniela ReiderDepartment for Dermatology and Venereology, Medical University Innsbruck, Anichstraße 35, 6020, Innsbruck, Austria.
Felix NägeleSection for clinical and functional Anatomy, Medical University Innsbruck, Müllerstraße 59, 6020, Innsbruck, Austria.
Anne-Françoise SpinoitDepartment of Urology, University Clinic Gent, Corneel Heymanslaan 10, 9000, Gent, Belgium.
Elisabeth PechrigglDepartment of Plastic, Reconstructive and Aesthetic Surgery, Medical University Innsbruck, Innerkoflerstraße 1, Innsbruck, Austria.
Nikolaus RomaniDepartment for Dermatology and Venereology, Medical University Innsbruck, Anichstraße 35, 6020, Innsbruck, Austria.
Helga FritschSection for clinical and functional Anatomy, Medical University Innsbruck, Müllerstraße 59, 6020, Innsbruck, Austria.
Josef OswaldDepartment of Pediatric Urology, Hospital of the Sisters of Charity, Ordensklinikum Linz, Seilerstätte 4, 4020, Linz, Austria.
Innsbruck Medical University · ATAGO Austria · ATGhent University · BESisters of Charity Hospital · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHypospadias are among the most common genital malformations. Langerhans Cells (LCs) play a pivotal role in HIV and HPV infection. The migration of LC precursors to skin coincides with the embryonic period of hypospadias development and genetic alterations leading to the formation of hypospadias impact the development of ectodermally derived tissues. We hypothesized that this might be associated with a difference in frequency or morphology of epidermal and dermal LCs in hypospadias patients.

methodsA total of 43 patients from two centers were prospectively included into this study after parental consent and ethics approval. Epidermal and dermal sheets were prepared from skin samples of 26 patients with hypospadias, 13 patients without penile malformations and 4 patients with penile malformations other than hypospadias. Immunofluorescence staining of sheets was performed with anti-HLA-DR-FITC and anti-CD207/Langerin-A594 antibodies. Skin sections from 11 patients without penile malformation and 11 patients with hypospadias were stained for Langerin. Frequencies as well as morphology and distribution of epidermal and dermal LCs on sheets and sections were microscopically evaluated. Cell counts were compared by unpaired t-tests.

resultsThere was no difference in frequency of epidermal LCs, Neither on sheets (873 ± 61 vs. 940 ± 84LCs/mm

conclusionsLCs are present in similar frequencies and with a comparable morphology and distribution in patients with hypospadias as compared to children without penile malformations. This suggests that patients with hypospadias are not different from patients with normal penile development considering this particular compartment of their skin immunity.

Indexed as

Langerhans CellsAntigens, CDChild, PreschoolEpidermisHLA-DR AntigensHumansHypospadiasInfantLectins, C-TypeMaleMannose-Binding LectinsProspective StudiesSkinAntigens, CDCD207 protein, humanHLA-DR AntigensLectins, C-TypeMannose-Binding LectinsForeskinHIVHPVHypospadiasLangerhans cells

Identifiers

PMID31718599
PMCPMC6852928
OpenAlexW2985451455

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.