ArticleRapid communications in mass spectrometry : RCM2020
A novel caged Cookson-type reagent toward a practical vitamin D derivatization method for mass spectrometric analyses.
Article in Rapid communications in mass spectrometry : RCM, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 10 citations in OpenAlex.
- Evaluation of correlations between serum and urinary vitamin D metabolites using LC-MS/MS.Bioscience reports · 2026Article
- Development of free 25-hydroxyvitamin D3 assay method using liquid chromatography-tandem mass spectrometry.Bioscience reports · 2022Article
- Synthesis of Deuterium-Labeled Vitamin D Metabolites as Internal Standards for LC-MS Analysis.Molecules (Basel, Switzerland) · 2022Article
- A novel caged Cookson-type reagent toward a practical vitamin D derivatization method for mass spectrometric analyses.Rapid communications in mass spectrometry : RCM · 2020Article
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Authors and funding
9 authors at 4 institutions in 1 country.
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Abstract
rationale25-Hydroxylated vitamin D is the best marker for vitamin D (VD). Due to its low ionization efficiency, a Cookson-type reagent, 1,2,4-triazoline-3,5-dione (TAD), is used to improve the detection/quantification of VD metabolites by liquid chromatography/tandem mass spectrometry (LC/MS/MS). However, the high reactivity of TAD makes its solution stability low and inconvenient for practical use. We here describe the development of a novel caged Cookson-type reagent, and we assess its performances in the quantitative and differential detection of four VD metabolites in serum using LC/MS/MS.
methodsCaged 4-(4'-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione (DAPTAD) analogues were prepared from 4-(4'-dimethylaminophenyl)-1,2,4-triazolidine-3,5-dione. Their stability and reactivity were examined. The optimized caged DAPTAD (14-(4-(dimethylamino)phenyl)-9-phenyl-9,10-dihydro-9,10-[1,2]epitriazoloanthracene-13,15-dione, DAP-PA) was used for LC/MS/MS analyses of VD metabolites.
resultsThe solution stability of DAP-PA in ethyl acetate dramatically improved compared with that of the non-caged one. We measured the thermal retro-Diels-Alder reaction enabling the release of DAPTAD and found that the derivatization reaction was temperature-dependent. We also determined the detection limit and the lower limit of quantifications for four VD metabolites with DAPTAD derivatization.
conclusionsDAP-PA was stable enough for mid- to long-term storage in solution. This advantage shall contribute to the detection and quantification of VD in clinical laboratories, and as such to the broader use of clinical mass spectrometry.
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