ArticlePloS one2019
CRISPR/Cas9 gene editing in the West Nile Virus vector, Culex quinquefasciatus Say.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
15 citing papers in PubMed.
- Generation of Cas9 Knock-InDNA · 2025Article
- Pioneering genome editing in parthenogenetic stick insects: CRISPR/Cas9-mediated gene knockout in Medauroidea extradentata.Scientific reports · 2025Article
- Manipulation of a New Non-model Insect Genome Using Targeted CRISPR-Era Approaches.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A multiplexed, confinable CRISPR/Cas9 gene drive can propagate in caged Aedes aegypti populations.Nature communications · 2024Article
- CRISPR-based gene drives generate super-Mendelian inheritance in the disease vector Culex quinquefasciatus.Nature communications · 2023Article
- Recognition of Arboviruses by the Mosquito Immune System.Biomolecules · 2023Review
- CRISPR-based gene drives generate super-Mendelian inheritance in the disease vectorbioRxiv : the preprint server for biology · 2023Article
- Maternally Instigated Diapause inFrontiers in behavioral neuroscience · 2022Review
- Evaluation of Gene Knockouts by CRISPR as Potential Targets for the Genetic Engineering of the MosquitoThe CRISPR journal · 2021Article
- CRISPR/Cas-9 mediated knock-in by homology dependent repair in the West Nile Virus vector Culex quinquefasciatus Say.Scientific reports · 2021Article
- Suppressed expression of oxidoreductin-like protein, Oxidor, increases follicle degeneration and decreases survival during the overwintering diapause of the mosquito Culex pipiens.Comparative biochemistry and physiology. Part A, Molecular & integrative physiology · 2021Article
- Optimized CRISPR tools and site-directed transgenesis towards gene drive development in Culex quinquefasciatus mosquitoes.Nature communications · 2021Article
- Targeting female flight for genetic control of mosquitoes.PLoS neglected tropical diseases · 2020Article
- Ommochrome pathway genes kynurenine 3-hydroxylase and cardinal participate in eye pigmentation in Plutella xylostella.BMC molecular and cell biology · 2020Article
- Highlights in Medical Entomology, 2019: Familiar Foes and New Frontiers.Journal of medical entomology · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Culex quinquefasciatus Say is an opportunistic blood feeder with a wide geographic distribution which is also a major vector for a range of diseases of both animals and humans. CRISPR/Cas technologies have been applied to a wide variety of organisms for both applied and basic research purposes. CRISPR/Cas methods open new possibilities for genetic research in non-model organisms of public health importance. In this work we have adapted microinjection techniques commonly used in other mosquito species to Culex quinquefasciatus, and have shown these to be effective at generating homozygous knock-out mutations of a target gene in one generation. This is the first description of the kmo gene and mutant phenotype in this species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.