ArticleNucleic acids research2019
Rapid in vitro production of single-stranded DNA.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- HAMNO and CGK733 as BRCAness-driven candidates exhibit synthetic lethality with olaparib in BRCA-proficient ovarian cancer.NPJ precision oncology · 2026Article
- Long-stranded XNA-cssDNA hybrids for robust data storage.Science advances · 2026Article
- In-Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and Testing.bioRxiv : the preprint server for biology · 2026Article
- Biosensor Based on pSi-SU-8-GQD Heterostructures for Enhanced Viral Detection by Dual Signal Amplification Strategy.ACS omega · 2026Article
- Machine learning-optimized long single-stranded DNA synthesis technology empowers high-precision diagnostic-therapeutic integration in living cells.Nucleic acids research · 2026Article
- Versatile and Selective Biomolecule Pulldown with Combinatorial DNA-Crosslinked Polymers.Angewandte Chemie (International ed. in English) · 2026Article
- Expedient production of site specifically nucleobase-labelled or hypermodified RNA with engineered thermophilic DNA polymerases.Nature communications · 2024Article
- A method for generating user-defined circular single-stranded DNA from plasmid DNA using Golden Gate intramolecular ligation.Biotechnology and bioengineering · 2023Article
- Efficient precise integration of large DNA sequences with 3'-overhang dsDNA donors using CRISPR/Cas9.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Evaluation of Nonmodified Wireframe DNA Origami for Acute Toxicity and Biodistribution in Mice.ACS applied bio materials · 2023Article
- Evaluation of non-modified wireframe DNA origami for acute toxicity and biodistribution in mice.bioRxiv : the preprint server for biology · 2023Article
- Functionalizing DNA origami to investigate and interact with biological systems.Nature reviews. Materials · 2023Article
- Gene Editing in Mouse Zygotes Using the CRISPR/Cas9 System.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Design, Assembly, and Function of DNA Origami Mechanisms.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Efficient Homology-Directed Repair with Circular Single-Stranded DNA Donors.The CRISPR journal · 2022Article
- PCR Methods for the Generation of Catalytic ssDNA.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Review
- Mini review: Enzyme-based DNA synthesis and selective retrieval for data storage.Computational and structural biotechnology journal · 2021Review
- Opportunities, Barriers, and a Strategy for Overcoming Translational Challenges to Therapeutic Nucleic Acid Nanotechnology.ACS nano · 2020Article
- Synthesis of DNA Origami Scaffolds: Current and Emerging Strategies.Molecules (Basel, Switzerland) · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 3 institutions in 1 country.
Funding
Abstract
There is increasing demand for single-stranded DNA (ssDNA) of lengths >200 nucleotides (nt) in synthetic biology, biological imaging and bionanotechnology. Existing methods to produce high-purity long ssDNA face limitations in scalability, complexity of protocol steps and/or yield. We present a rapid, high-yielding and user-friendly method for in vitro production of high-purity ssDNA with lengths up to at least seven kilobases. Polymerase chain reaction (PCR) with a forward primer bearing a methanol-responsive polymer generates a tagged amplicon that enables selective precipitation of the modified strand under denaturing conditions. We demonstrate that ssDNA is recoverable in ∼40-50 min (time after PCR) with >70% yield with respect to the input PCR amplicon, or up to 70 pmol per 100 μl PCR reaction. We demonstrate that the recovered ssDNA can be used for CRISPR/Cas9 homology directed repair in human cells, DNA-origami folding and fluorescent in-situ hybridization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.