Evidence map›Paper›PMID 31712791›Full record

ArticleLab on a chip2020

Injection molded open microfluidic well plate inserts for user-friendly coculture and microscopy.

John H Day, Tristan M Nicholson, Xiaojing Su, Tammi L van Neel, Ivor Clinton, Anbarasi Kothandapani, Jinwoo Lee, Max H Greenberg, John K Amory, Thomas J Walsh and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Lab on a chip, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Interfacial tension driven open droplet microfluidics.Advanced materials interfaces · 2023
    Article
  2. Article
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

John H DayDepartment of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, USA. abt1@uw.edu.ORCID 0000-0002-5515-6606
Tristan M NicholsonDepartment of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, USA. abt1@uw.edu and Department of Urology, University of Washington School of Medicine, Seattle, WA 98195, USA.
Xiaojing SuDepartment of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, USA. abt1@uw.edu.ORCID 0000-0002-0174-2327
Tammi L van NeelDepartment of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, USA. abt1@uw.edu.ORCID 0000-0002-3626-8418
Ivor ClintonDepartment of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, USA. abt1@uw.edu.
Anbarasi KothandapaniDepartment of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI 53706, USA.ORCID 0000-0001-9701-0800
Jinwoo LeeDepartment of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI 53706, USA and Department of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Max H GreenbergDepartment of Surgery, NorthShore University Research Institute, Affiliate of University of Chicago Pritzker School of Medicine, Evanston, IL 60201, USA.
John K AmoryDepartment of Medicine, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-7419-435X
Thomas J WalshDepartment of Urology, University of Washington School of Medicine, Seattle, WA 98195, USA.
Charles H MullerDepartment of Urology, University of Washington School of Medicine, Seattle, WA 98195, USA and Male Fertility Laboratory, Department of Urology, University of Washington School of Medicine, Seattle, WA 98195, USA.
Omar E FrancoDepartment of Surgery, NorthShore University Research Institute, Affiliate of University of Chicago Pritzker School of Medicine, Evanston, IL 60201, USA.
Colin R JefcoateDepartment of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Susan E CrawfordDepartment of Surgery, NorthShore University Research Institute, Affiliate of University of Chicago Pritzker School of Medicine, Evanston, IL 60201, USA.
Joan S JorgensenDepartment of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI 53706, USA.ORCID 0000-0002-5787-8856
Ashleigh B ThebergeDepartment of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, USA. abt1@uw.edu and Department of Urology, University of Washington School of Medicine, Seattle, WA 98195, USA.ORCID 0000-0002-3394-1435

Funding

Theberge Admin Supp Undergraduate Summer Research ExperiencesR35GM128648 · NIGMS · UNIVERSITY OF WASHINGTON · PI Ashleigh Brooks Theberge · 2018 to 2026
$4.7M
Risk-stratification of Prostate Cancers via Field Carcinogenesis NanocytologyR01CA200064 · NCI · NORTHWESTERN UNIVERSITY · PI BACKMAN, VADIM, CRAWFORD, SUSAN E. · 2015 to 2019
$3.1M
Mediators for dynamic regulation of Star transcription in Leydig cellsR01HD090660 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI JEFCOATE, COLIN ROBERT, JORGENSEN, JOAN S · 2017 to 2021
$3.1M
Functional Consequences of Racially Associated Ephrin Alterations on the Prostate Tumor MicroenvironmentR01CA242920 · NCI · LOUISIANA STATE UNIV HSC SHREVEPORT · PI FRANCO CORONEL, OMAR · 2019 to 2024
$1.8M
NCI NIH HHS R01 CA200064NCI NIH HHS R01 CA242920NICHD NIH HHS R01 HD090660NIGMS NIH HHS R35 GM128648
6 · The paper itself

Abstract

Open microfluidic cell culture systems are powerful tools for interrogating biological mechanisms. We have previously presented a microscale cell culture system, based on spontaneous capillary flow of biocompatible hydrogels, that is integrated into a standard cell culture well plate, with flexible cell compartment geometries and easy pipet access. Here, we present two new injection molded open microfluidic devices that also easily insert into standard cell culture well plates and standard culture workflows, allowing seamless adoption by biomedical researchers. These platforms allow culture and study of soluble factor communication among multiple cell types, and the microscale dimensions are well-suited for rare primary cells. Unique advances include optimized evaporation control within the well, manufacture with reproducible and cost-effective rapid injection molding, and compatibility with sample preparation workflows for high resolution microscopy (following well-established coverslip mounting procedures). In this work, we present several use cases that highlight the usability and widespread utility of our platform including culture of limited primary testis cells from surgical patients, microscopy readouts including immunocytochemistry and single molecule fluorescence in situ hybridization (smFISH), and coculture to study interactions between adipocytes and prostate cancer cells.

Indexed as

Lab-On-A-Chip DevicesCells, CulturedCell SurvivalCoculture TechniquesHumansImmunohistochemistryIn Situ Hybridization, FluorescenceMaleTestis

Identifiers

PMID31712791
PMCPMC6917835

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.