ArticleScientific reports2019
Stability of cytoplasmic nanoviscosity during cell cycle of HeLa cells synchronized with Aphidicolin.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Reversible and effective cell cycle synchronization method for studying stage-specific processes.Life science alliance · 2025Article
- Reversible and effective cell cycle synchronization method for studying stage-specific investigations.bioRxiv : the preprint server for biology · 2024Article
- Physicochemical Perspective of Biological Heterogeneity.ACS physical chemistry Au · 2024Review
- Living Cell as a Self-Synchronized Chemical Reactor.The journal of physical chemistry letters · 2024Review
- Nanomolar Nitric Oxide Concentrations in Living Cells Measured by Means of Fluorescence Correlation Spectroscopy.Molecules (Basel, Switzerland) · 2022Article
- Cell Cycle Synchronization of Primary and Cultured Articular Chondrocytes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Poly(A)+ Sensing of Hybridization-Sensitive Fluorescent Oligonucleotide Probe Characterized by Fluorescence Correlation Methods.International journal of molecular sciences · 2021Article
- Nanoscale Viscosity of Cytoplasm Is Conserved in Human Cell Lines.The journal of physical chemistry letters · 2020Article
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Authors and funding
3 authors.
Funding
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Abstract
Nanoviscosity of the cytoplasm is a key factor affecting diffusion of biomolecules and - as a consequence - rates of biochemical reactions in a cell. Nanoviscosity is an outcome of variable chemical and structural factors, which can temporarily change with cell-cycle associated changes of intracellular architecture. Thus, the question arises, whether rates of biochemical reactions depend on the point of cell cycle. In this paper we address this topic by constant observation of nanoviscosity of HeLa cells cytoplasm during S, G2 and G1 phases after Aphidicolin synchronization. For this purpose we measured diffusion rates of EGFP molecules using fluorescence correlation spectroscopy (FCS). To our surprise, a counter-intuitive stability of cytoplasmic viscosity was observed during the cell cycle. Our results hint at possible existence of robust mechanism maintaining stable physiological viscosity of the cytoplasm, despite huge structural changes during cell cycle.
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