Evidence map›Paper›PMID 31712282›Full record

ArticleJournal of bacteriology2020

Elevated Expression of a Functional Suf Pathway in Escherichia coli BL21(DE3) Enhances Recombinant Production of an Iron-Sulfur Cluster-Containing Protein.

Elliot I Corless, Erin L Mettert, Patricia J Kiley, Edwin Antony

Open access · greenAbstract read
In one paragraph

Article in Journal of bacteriology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 45 citations in OpenAlex.

  1. Review
  2. Improving cell-free metabolism through direct integration of artificial respiratory chains.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Journal of the American Chemical Society · 2025
    Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Proteomic strategies to interrogate the Fe-S proteome.Biochimica et biophysica acta. Molecular cell research · 2024
    Review
  12. Article
  13. FromApplied and environmental microbiology · 2024
    Article
  14. Article
  15. Maturation strategy influences expression levels and cofactor occupancy in Fe-S proteins.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2023
    Article
  16. Article
  17. Article
  18. Heterologous Expression of ActiveApplied and environmental microbiology · 2022
    Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Elliot I CorlessDepartment of Biological Sciences, Marquette University, Milwaukee, Wisconsin, USA.
Erin L MettertDepartment of Biomolecular Chemistry, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Patricia J KileyDepartment of Biomolecular Chemistry, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin, USA pjkiley@wisc.edu edwin.antony@health.slu.edu.
Edwin AntonyDepartment of Biological Sciences, Marquette University, Milwaukee, Wisconsin, USA pjkiley@wisc.edu edwin.antony@health.slu.edu.
University of Wisconsin–Madison · USMarquette University · USSaint Louis University · US

Funding

Bacterial anaerobic iron homeostasisR01GM115894 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI KILEY, PATRICIA J · 2015 to 2018
$1.2M
NIGMS NIH HHS R01 GM115894
6 · The paper itself

Abstract

Structural and spectroscopic analysis of iron-sulfur [Fe-S] cluster-containing proteins is often limited by the occupancy and yield of recombinantly produced proteins. Here we report that

Indexed as

Adenosine TriphosphatasesBiosynthetic PathwaysCarrier ProteinsEscherichia coliEscherichia coli ProteinsIron-Sulfur ProteinsOperonAdenosine TriphosphatasesCarrier ProteinsEscherichia coli ProteinsIron-Sulfur ProteinsSufA protein, E coliSufB protein, E coliSufC protein, E coliSufD protein, E coliSuf E protein, E coliE. coli BFe-S cluster biogenesisFe-S protein overexpressionFe-S protein overproductioniron-sulfur biogenesisSufSuf pathway

Identifiers

PMID31712282
PMCPMC6964742
OpenAlexW2989209102

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.