ArticleBMC plant biology2019
Isolation, purification and characterization of an ascorbate peroxidase from celery and overexpression of the AgAPX1 gene enhanced ascorbate content and drought tolerance in Arabidopsis.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 81 citations in OpenAlex.
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- A truncated B-box zinc finger transcription factor confers drought sensitivity in modern cultivated tomatoes.Nature communications · 2024Article
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- The ascorbate-glutathione cycle coming of age.Journal of experimental botany · 2024Review
- Transcriptome Profiling of a Salt Excluder Hybrid Grapevine Rootstock 'Ruggeri' throughout Salinity.Plants (Basel, Switzerland) · 2024Article
- Genome-wide identification and analysis of ascorbate peroxidase (APX) gene family in hemp (PeerJ · 2024Article
- Uncovering the Role of Hormones in Enhancing Antioxidant Defense Systems in Stressed Tomato (Plants (Basel, Switzerland) · 2023Review
- Foliar Selenium Application to Reduce the Induced-Drought Stress Effects in Coffee Seedlings: Induced Priming or Alleviation Effect?Plants (Basel, Switzerland) · 2023Article
- Comparative transcriptome analysis of Saposhnikovia divaricata to reveal drought and rehydration adaption strategies.Molecular biology reports · 2023Article
- A prophage-encoded effector from "Candidatus Liberibacter asiaticus" targets ASCORBATE PEROXIDASE6 in citrus to facilitate bacterial infection.Molecular plant pathology · 2023Article
- AgMYB5, an MYB transcription factor from celery, enhanced β-carotene synthesis and promoted drought tolerance in transgenic Arabidopsis.BMC plant biology · 2023Article
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7 authors at 1 institution in 1 country.
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Abstract
backgroundCelery is a widely cultivated vegetable abundant in ascorbate (AsA), a natural plant antioxidant capable of scavenging free radicals generated by abiotic stress in plants. Ascorbate peroxidase (APX) is a plant antioxidant enzyme that is important in the synthesis of AsA and scavenging of excess hydrogen peroxide. However, the characteristics and functions of APX in celery remain unclear to date.
resultsIn this study, a gene encoding APX was cloned from celery and named AgAPX1. The transcription level of the AgAPX1 gene was significantly upregulated under drought stress. AgAPX1 was expressed in Escherichia coli BL21 (DE3) and purified. The predicted molecular mass of rAgAPX1 was 33.16 kDa, which was verified by SDS-PAGE assay. The optimum pH and temperature for rAgAPX1 were 7.0 and 55 °C, respectively. Transgenic Arabidopsis hosting the AgAPX1 gene showed elevated AsA content, antioxidant capacity and drought resistance. Less decrease in net photosynthetic rate, chlorophyll content, and relative water content contributed to the high survival rate of transgenic Arabidopsis lines after drought.
conclusionsThe characteristics of APX in celery were different from that in other species. The enhanced drought resistance of overexpressing AgAPX1 in Arabidopsis may be achieved by increasing the accumulation of AsA, enhancing the activities of various antioxidant enzymes, and promoting stomatal closure. Our work provides new evidence to understand APX and its response mechanisms to drought stress in celery.
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