Evidence map›Paper›PMID 31710731›Full record

ArticleTransplant international : official journal of the European Society for Organ Transplantation2020

Quantitative PCR of INDELs to measure donor-derived cell-free DNA-a potential method to detect acute rejection in kidney transplantation: a pilot study.

Eva-Maria Dauber, Dagmar Kollmann, Nicolas Kozakowski, Susanne Rasoul-Rockenschaub, Thomas Soliman, Gabriela A Berlakovich, Wolfgang R Mayr

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transplant international : official journal of the European Society for Organ Transplantation, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 2 pooled it
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 2 syntheses or guidelines pooled it, 38 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Eva-Maria DauberDepartment of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, Wien, Austria.ORCID 0000-0003-0285-9540
Dagmar KollmannDivision of Transplantation, Department of Surgery, Medical University of Vienna, Wien, Austria.ORCID 0000-0002-7417-237X
Nicolas KozakowskiDepartment of Pathology, Medical University of Vienna, Wien, Austria.
Susanne Rasoul-RockenschaubDivision of Transplantation, Department of Surgery, Medical University of Vienna, Wien, Austria.
Thomas SolimanDivision of Transplantation, Department of Surgery, Medical University of Vienna, Wien, Austria.
Gabriela A BerlakovichDivision of Transplantation, Department of Surgery, Medical University of Vienna, Wien, Austria.
Wolfgang R MayrDepartment of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, Wien, Austria.
Medical University of Vienna · ATMetropolitan University · BD

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The quantification of donor-derived cell-free DNA (ddcfDNA) in recipient's plasma is a novel, but technically challenging noninvasive method to assist the diagnosis of acute rejection (AR). A quantitative real-time PCR (qPCR) approach targeting insertion/deletion polymorphisms (INDEL) was adapted to measure ddcfNA in plasma samples from 29 kidney transplant recipients obtained at time of clinically indicated biopsies (eight patients with a histologically verified AR, nine with borderline rejection and 12 without evidence of rejection). Measured ddcfDNA levels of smaller INDEL amplicon targets differed significantly (P = 0.016, Kruskal-Wallis H test) between recipients with biopsy-proven AR (median 5.24%; range 1.00-9.03), patients without (1.50%; 0.41-6.50) and patients with borderline AR (1.91%; 0.58-5.38). Similarly, pairwise testing by Mann-Whitney U-tests revealed significant differences between recipients with AR and without AR (P = 0.012) as well as patients with AR and borderline histology (P = 0.015). Receiver operating characteristic (ROC) analysis revealed an area under the ROC curve for discriminating AR and non-AR biopsies of 0.84 (95% CI: 0.66-1.00). The determined cutoff value of 2.7% ddcfDNA showed a sensitivity of 0.88 (95% CI: 0.63-1.00) and specificity of 0.81 (95% CI: 0.64-0.98). INDEL qPCR represents a novel method to quantify ddcfDNA on standard qPCR instruments within 6-8 h with high sensitivity and specificity to detect AR.

Indexed as

Cell-Free Nucleic AcidsKidney TransplantationBiomarkersGraft RejectionHumansPilot ProjectsReal-Time Polymerase Chain ReactionBiomarkersCell-Free Nucleic Acidsacute rejectioncell-free DNAdonor-derived cell-free DNAINDELqPCR

Identifiers

PMID31710731
PMCPMC7065216
OpenAlexW2984097508

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.