ArticlePloS one2019
Discovery of powdery mildew resistance gene candidates from Aegilops biuncialis chromosome 2Mb based on transcriptome sequencing.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 23 citations in OpenAlex.
- Integrative approach to identify robust pattern recognition receptors in Eucalyptus grandis: novel candidates for disease resistance.Plant molecular biology · 2026Article
- RNA-Seq-based transcriptomics reveals differential gene expression between two Pisum sativum subspecies and uncovers their molecular marker profiles.BMC genomics · 2025Article
- A linkage map of Aegilops biuncialis reveals significant genomic rearrangements compared to bread wheat.The plant genome · 2025Article
- DArTseq genotyping facilitates identification of Aegilops biuncialis chromatin introgressed into bread wheat Mv9kr1.Plant molecular biology · 2024Article
- A Cluster ofPlants (Basel, Switzerland) · 2024Article
- Chromosome genomics facilitates the marker development and selection of wheat-Aegilops biuncialis addition, substitution and translocation lines.Scientific reports · 2023Article
- Genomic characterization and gene bank curation ofFrontiers in plant science · 2023Article
- Mapping of the novel powdery mildew resistance gene Pm2Mb from Aegilops biuncialis based on ph1b-induced homoeologous recombination.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Identification of New QTLs for Dietary Fiber Content inInternational journal of molecular sciences · 2022Article
- Transcriptome Profiling to the Effects of Drought Stress on Different Propagation Modes of Tea Plant (Frontiers in genetics · 2022Article
- Combating powdery mildew: Advances in molecular interactions betweenFrontiers in plant science · 2022Review
- RNA-Seq-based DNA marker analysis of the genetics and molecular evolution of Triticeae species.Functional & integrative genomics · 2021Review
- Genotype-Specific Expression and NLR Repertoire Contribute to Phenotypic Resistance Diversity inFrontiers in plant science · 2021Article
- Molecular Cytogenetic Identification of Wheat-International journal of molecular sciences · 2020Article
- Physical Mapping ofInternational journal of molecular sciences · 2020Article
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Authors and funding
8 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Powdery mildew is one of the most widespread diseases of wheat. The development and deployment of resistant varieties are one of the most economical and effective methods to manage this disease. Our previous study showed that the gene(s) at 2Mb in Chinese Spring (CS)-Aegilops biuncialis 2Mb disomic addition line TA7733 conferred a high level of resistance to powdery mildew of wheat. In this study, resistance spectrum of TA7733 was assayed by using 15 Blumeria graminis f. sp. tritici (Bgt) isolates prevalent in different regions of China. The result indicated that TA7733 was highly resistant to all tested Bgt isolates and the gene(s) on chromosome 2Mb conferred broad-spectrum resistance to powdery mildew. In order to characterize mechanism of powdery mildew resistance by identifying candidates R-genes derived from Ae. biuncialis chromosome 2Mb and develop 2Mb-specific molecular markers, we performed RNA-seq analysis on TA7733 and CS. In total we identified 7,278 unigenes that showed specific expression in TA7733 pre and post Bgt-infection when compared to CS. Of these 7,278 unigenes, 295 were annotated as putative resistance (R) genes. Comparatively analysis of R-gene sequences from TA7733 and CS and integration CS Ref Seq v1.0 were used to develop R-gene specific primers. Of 295 R-genes we identified 53 R-genes were specific to 2Mb and could be involved in powdery mildew resistance. Functional annotation of majority of the 53 R-genes encoded nucleotide binding leucine rich repeat (NLR) protein. The broad-spectrum resistance to powdery mildew in TA7733 and availability of 2Mb-derived putative candidate R-gene specific molecular markers identified in this study will lay foundations for transferring powdery mildew resistance from 2Mb to common wheat by inducing CS-Ae. biuncialis homoeologous recombination. Our study also provides useful candidates for further isolation and cloning of powdery mildew resistance gene(s) from Ae. biuncialis chromosome 2Mb.
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