Evidence map›Paper›PMID 31710293›Full record

ArticleeLife2019

Cryo electron tomography with volta phase plate reveals novel structural foundations of the 96-nm axonemal repeat in the pathogen

Simon Imhof, Jiayan Zhang, Hui Wang, Khanh Huy Bui, Hoangkim Nguyen, Ivo Atanasov, Wong H Hui, Shun Kai Yang, Z Hong Zhou, Kent L Hill

Open access · goldAbstract read
In one paragraph

Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.

0numbers the graph read from it
0cells of the map it votes in
43citing papers in PubMed
5.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

43 citing papers in PubMed, 74 citations in OpenAlex.

  1. Article
  2. Axonemal dynein contributions to flagellar beat types and waveforms.bioRxiv : the preprint server for biology · 2025
    Article
  3. Heterogeneity of radial spoke components in Tetrahymena cilia.Cellular and molecular life sciences : CMLS · 2025
    Article
  4. Article
  5. CryoEM structure of thebioRxiv : the preprint server for biology · 2025
    Article
  6. Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. FAP20 is required for flagellum assembly inMolecular biology of the cell · 2024
    Article
  14. Article
  15. Article
  16. Article
  17. FAP20 is required for flagellum assembly inbioRxiv : the preprint server for biology · 2024
    Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Simon Imhof *Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, United States.ORCID 0000-0001-7514-6811
Jiayan Zhang *Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, United States.ORCID 0000-0003-3602-1199
Hui WangDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, United States.ORCID 0000-0002-9922-7170
Khanh Huy BuiDepartment of Anatomy and Cell Biology, McGill University, Montreal, United States.ORCID 0000-0003-2814-9889
Hoangkim NguyenDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, United States.
Ivo AtanasovCalifornia NanoSystems Institute, University of California, Los Angeles, Los Angeles, United States.
Wong H HuiCalifornia NanoSystems Institute, University of California, Los Angeles, Los Angeles, United States.
Shun Kai YangDepartment of Anatomy and Cell Biology, McGill University, Montreal, United States.
Z Hong ZhouDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, United States.ORCID 0000-0002-8373-4717
Kent L HillDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, United States.ORCID 0000-0001-6529-1273
California NanoSystems Institute · USUniversity of California, Los Angeles · US

Funding

UCLA NIGMS T32 Program AssessmentT32GM007185 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TORRES, JORGE · 1985 to 2020
$21.7M
Biochemical and Functional Analysis of TrypaninR01AI052348 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HILL, KENT L · 2002 to 2023
$7.4M
High-Resolution CryoEM Reconstruction of Large ComplexesR01GM071940 · NIGMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI ZHOU, Z HONG · 2006 to 2025
$5.0M
West/Midwest Consortium for High-Resolution Cryo Electron MicroscopyU24GM116792 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AGBANDJE-MCKENNA, MAVIS, DOKLAND, TERJE · 2016 to 2020
$3.0M
HIGH-END CRYOEM INSTRUMENT FOR UCLA ELECTRON IMAGING CTR FOR NANOMACHINES: AIDSS10RR023057 · NCRR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHOU, Z HONG · 2006 to 2006
$1.6M
Direct Detection Device for atomic resolution cryoEM of macromolecular complexesS10OD018111 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHOU, Z HONG · 2014 to 2014
$598k
Biochemical and Functional Analysis of TrypaninR56AI052348 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HILL, KENT L · 2007 to 2007
$374k
National Science Foundation DBI-1338135National Science Foundation DMR-1548924NCRR NIH HHS S10 RR023057NIAID NIH HHS R01 AI052348NIAID NIH HHS R56 AI052348NIGMS NIH HHS R01 GM071940NIGMS NIH HHS T32 GM007185NIGMS NIH HHS U24 GM116792NIH HHS AI052348NIH HHS GM007185NIH HHS R01GM071940NIH HHS S10 OD018111NIH HHS S10OD018111NIH HHS S10RR23057NIH HHS U24GM116792Swiss National Science Foundation P2BEP3_162094Swiss National Science Foundation P300PA_174358
6 · The paper itself

Abstract

The 96-nm axonemal repeat includes dynein motors and accessory structures as the foundation for motility of eukaryotic flagella and cilia. However, high-resolution 3D axoneme structures are unavailable for organisms among the Excavates, which include pathogens of medical and economic importance. Here we report cryo electron tomography structures of the 96-nm repeat from

Indexed as

AxonemeCryoelectron MicroscopyElectron Microscope TomographyImaging, Three-DimensionalProtein BindingProtozoan ProteinsTrypanosoma brucei bruceiProtozoan Proteinsaxonemecell biologyciliumdyneininfectious diseasemicrobiologymotilityparasitetrypanosome

Identifiers

PMID31710293
PMCPMC6974359
OpenAlexW2987293451

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.