Evidence map›Paper›PMID 31709750›Full record

Observational studyCancer medicine2020

Unbiased enrichment of urine exfoliated cells on nanostructured substrates for sensitive detection of urothelial tumor cells.

Xin Wang, Yuanyuan Gu, Shiwei Zhang, Gangqiang Li, Tianyao Liu, Tianwei Wang, Haixiang Qin, Bo Jiang, Lin Zhu, Yajun Li and 6 more

Open access · goldAbstract readObservational Study
PubMed Full text at PMC Publisher
In one paragraph

Observational study in Cancer medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 5 institutions in 3 countries.

Xin WangDepartment of Urology, Drum Tower Hospital, Medical School of Nanjing University, Institute of Urology, Nanjing University, Nanjing, China.ORCID 0000-0002-9018-4653
Yuanyuan GuPerMed Biomedicine Institute, Shanghai, China.ORCID 0000-0002-3790-1983
Shiwei ZhangDepartment of Urology, Drum Tower Hospital, Medical School of Nanjing University, Institute of Urology, Nanjing University, Nanjing, China.
Gangqiang LiDepartment of Pathology, Naval Characteristic Medical Center, Shanghai, China.
Tianyao LiuDepartment of Urology, Drum Tower Hospital, Medical School of Nanjing University, Institute of Urology, Nanjing University, Nanjing, China.
Tianwei WangDepartment of Urology, Drum Tower Hospital, Medical School of Nanjing University, Institute of Urology, Nanjing University, Nanjing, China.
Haixiang QinDepartment of Urology, Drum Tower Hospital, Medical School of Nanjing University, Institute of Urology, Nanjing University, Nanjing, China.
Bo JiangDepartment of Urology, Drum Tower Hospital, Medical School of Nanjing University, Institute of Urology, Nanjing University, Nanjing, China.
Lin ZhuPerMed Biomedicine Institute, Shanghai, China.
Yajun LiPerMed Biomedicine Institute, Shanghai, China.
Haozhi LeiPerMed Biomedicine Institute, Shanghai, China.
Ming LiDepartment of Pathology, The Affiliated Suzhou Municipal Hospital of Nanjing Medical University, Suzhou, China.
Qun ZhangPerMed Biomedicine Institute, Shanghai, China.
Rong YangDepartment of Urology, Drum Tower Hospital, Medical School of Nanjing University, Institute of Urology, Nanjing University, Nanjing, China.
Feng FangDepartment of Pharmacology, Nanjing Medical University, Nanjing, China.
Hongqian GuoDepartment of Urology, Drum Tower Hospital, Medical School of Nanjing University, Institute of Urology, Nanjing University, Nanjing, China.
Nanjing Drum Tower Hospital · CNInstitute for Biomedicine · ITNanjing Medical University · CNNaval Medical Research Command · USSuzhou Municipal Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEarly detection of urothelial carcinoma (UC) by noninvasive diagnostic methods with high accuracy is still underscored. This study aimed to develop a noninvasive assay incorporating both enrichment of urine exfoliated cells and immunoassays for UC detection.

methodsPolystyrene dishes were exposed to oxygen plasma and modified with 3-aminopropyltriethoxysilane to prepare amine-functionalized nanostructured substrates (NS). Performance characterization of NS was evaluated by atomic force microscope and X-ray photoelectron spectroscopy. Urine exfoliated cells were captured by NS and then immunostained to detect urinary tumor cells (UTCs), which was called UTC assay. The receiver operating characteristic (ROC) curve, area under ROC curve (AUC), and Youden index were used to find the cutoff value of UTC assay. ROC analysis and McNemar test were used to compare the diagnostic accuracy of UTC assay with cytology. Kappa test was used to analyze the agreement of UTC assay and cytology with pathological diagnosis.

resultsNanostructured substrates had good cell binding yields of nucleated cells and tumor cells. CK20

conclusionsNanostructured substrates could be used to enrich the exfoliated cells from urine samples. UTC assay with NS has the potential to play a role in UC detection. The value of this assay still needs additional validation by large, multi-center studies.

Indexed as

AdultAgedAged, 80 and overBiomarkers, TumorCarcinoma, Transitional CellCell Line, TumorCell SeparationEarly Detection of CancerFeasibility StudiesFemaleHumansImmunoassayLiquid BiopsyMaleMicroscopy, Atomic ForceMiddle AgedBiomarkers, Tumorcytologyimmunocytologynanostructured substratesurinary tumor cellsurothelial carcinoma

Identifiers

PMID31709750
PMCPMC6943141
OpenAlexW2984905861

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.