ArticleNephrology (Carlton, Vic.)2020
Proteomics of human glomerulonephritis by laser microdissection and liquid chromatography-tandem mass spectrometry.
Article in Nephrology (Carlton, Vic.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- The current use of proteomics and metabolomics in glomerulonephritis: a systematic literature review.Journal of nephrology · 2024Pooled it
- Treatment strategies for rituximab-resistant primary membranous nephropathy: from resistance mechanisms to emerging therapies.Frontiers in immunology · 2026Review
- A case of crystalglobulin-induced nephropathy wherein M protein was identified by mass spectrometry and immunoglobulin G subclass staining.CEN case reports · 2025Article
- Review
- Lipoprotein glomerulopathy with markedly increased arterial stiffness successfully treated with a combination of fenofibrate and losartan: a case report.BMC nephrology · 2024Article
- Spatial Proteomics Reveals Alcohol-Induced Damages to the Crypts and Villi of the Mouse Small Intestine.Journal of proteome research · 2024Article
- Future landscape for the management of membranous nephropathy.Clinical kidney journal · 2023Review
- Comparative proteomic analysis of glomerular proteins in IgA nephropathy and IgA vasculitis with nephritis.Clinical proteomics · 2023Article
- Treatment of membranous nephropathy: Perspectives on current and future therapies.Frontiers in nephrology · 2023Review
- Apolipoprotein E-associated Lipoprotein Glomerulo-tubulopathy.Internal medicine (Tokyo, Japan) · 2023Article
- Mouse Paneth Cell-Enriched Proteome Enabled by Laser Capture Microdissection.Journal of proteome research · 2022Article
- Comparative proteomic analysis of glomerular proteins in primary and bucillamine-induced membranous nephropathy.Clinical proteomics · 2022Article
- Applications of spatially resolved omics in the field of endocrine tumors.Frontiers in endocrinology · 2022Review
- Renal deposition and clearance of recombinant poly-IgA complexes in a model of IgA nephropathy.The Journal of pathology · 2021Article
- Mechanisms of Primary Membranous Nephropathy.Biomolecules · 2021Review
- Renal diseases and the role of complement: Linking complement to immune effector pathways and therapeutics.Advances in immunology · 2021Review
- Differentiating Staphylococcus infection-associated glomerulonephritis and primary IgA nephropathy: a mass spectrometry-based exploratory study.Scientific reports · 2020Article
- Proteomics of human glomerulonephritis by laser microdissection and liquid chromatography-tandem mass spectrometry.Nephrology (Carlton, Vic.) · 2020Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimLaser microdissection (LMD) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) enable clinicians to analyse proteins from tissue sections. In nephrology, these methods are used to diagnose diseases of abnormal protein deposition, such as amyloidosis, but they are seldom applied to the diagnosis and pathophysiological understanding of human glomerular diseases.
methodsRenal biopsy specimens were obtained from five patients with IgA nephropathy (IgAN), five patients with membranous nephropathy (MN) and five kidney transplant donors (as controls). From 10-μm-thick sections of formalin-fixed, paraffin-embedded specimens, 0.3-mm
resultsFrom the control glomeruli, we identified more than 300 types of proteins. In patients with IgAN, we detected significant increases not only in IgA1 and in C3, but also in the factors related to oxidative stress and cell proliferation in comparison to the controls. In patients with MN, levels of IgG1, IgG4, C3, C4a and phospholipase-A2-receptor were significantly elevated in comparison to the controls, as were the aforementioned factors related to oxidative stress and cell proliferations detected in IgAN.
conclusionApplication of LMD and LC-MS/MS to renal biopsy specimens enabled us to identify not only pathognomonic proteins for the diagnosis, but also several factors possibly involved in the pathogenesis of human glomerular diseases.
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