Evidence map›Paper›PMID 31707481›Full record

ArticleAMB Express2019

An exploration of the rapid transformation method for Dunaliella salina system.

Guannan Song, Wan Wang, Lina Hu, Yu Liu, Aifang Li, Jingxia Du, Jiao Wang, Mengyuan Jia, Shuying Feng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in AMB Express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Grand Challenges in Microalgae Domestication.Frontiers in plant science · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Guannan SongMedical College, Henan University of Science and Technology, No. 263 Kaiyuan Avenue, Luoyang, 471023, Henan, China.
Wan WangMedical College, Henan University of Science and Technology, No. 263 Kaiyuan Avenue, Luoyang, 471023, Henan, China.
Lina HuMedical College, Henan University of Science and Technology, No. 263 Kaiyuan Avenue, Luoyang, 471023, Henan, China.
Yu LiuMedical College, Henan University of Science and Technology, No. 263 Kaiyuan Avenue, Luoyang, 471023, Henan, China.
Aifang LiMedical College, Henan University of Science and Technology, No. 263 Kaiyuan Avenue, Luoyang, 471023, Henan, China.
Jingxia DuMedical College, Henan University of Science and Technology, No. 263 Kaiyuan Avenue, Luoyang, 471023, Henan, China.
Jiao WangMedical College, Henan University of Science and Technology, No. 263 Kaiyuan Avenue, Luoyang, 471023, Henan, China.
Mengyuan JiaMedical College, Henan University of Science and Technology, No. 263 Kaiyuan Avenue, Luoyang, 471023, Henan, China.
Shuying FengMedical College, Henan University of Science and Technology, No. 263 Kaiyuan Avenue, Luoyang, 471023, Henan, China. fshy001@haust.edu.cn.

Funding

National Natural Science Foundation of China U1804112
6 · The paper itself

Abstract

As a new expression system, Dunaliella salina (D. salina) has bright prospects and applications in various fields. However, its application is currently restricted because of the low expression and instability of foreign gene in D. salina cells. During genetic operation, transformation is a crucial step for genes expression in D. salina system. Although several transformation methods are existing currently, many inherent deficiencies and limitations still can be found in actual practice. Thus, we attempted to set up a rapid transformation method using the change of salt concentrations for D. salina. Based on osmotic pressure difference, exogenous genes can be spontaneously transferred into D. salina cells. After that, transformed D. salina cells were subjected to histochemical and molecular analysis. The results showed that the reporter gene, beta-glucuronidase genes were successfully expressed in the positive transformants, and detected in all of transformed cells by PCR analysis. Moreover, different transformation parameters, containing the salt gradient, time, dye dosage and Triton X-100 concentration, were optimized to obtain an optimal transformation result. Taken together, we preliminarily established a rapid transformation method with the features of fast, simple, economic, and high-efficient. This method will provide a strong genetic manipulation tool for the future transformation of D. salina system.

Indexed as

Dunaliella salinaHigh efficiencyRapid methodSalt gradientTransformation

Identifiers

PMID31707481
PMCPMC6842366

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.