ArticleiScience2019
The Mechanosensitive Ion Channel Piezo1 Significantly Mediates In Vitro Ultrasonic Stimulation of Neurons.
Article in iScience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 126 papers, 1 of them a synthesis that pooled it.
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The trial behind it
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Who cites it
126 citing papers in PubMed, 1 synthesis or guideline pooled it, 269 citations in OpenAlex.
- Neuromodulation effects of low-intensity transcranial focused ultrasound in human, a systematic review focusing on motor and sensory functions.Journal of neuroengineering and rehabilitation · 2025Pooled it
- Exploring the role of neuro-immune modulation for psoriasis management: a narrative review of existing evidence and future therapies.Brain, behavior, & immunity - health · 2026Review
- Focused ultrasound neuromodulation for psychiatric disorders: a scoping review of clinical applications and current progress.Journal of neural transmission (Vienna, Austria : 1996) · 2026Review
- Sono-Mechanogenetics: Linking Ultrasound Physics With Cellular Mechanobiology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Soft Neural Interfaces for Circuit-Level Analysis of Magnetogenetic Deep Brain Stimulation in Parkinson's Disease Models.Advanced healthcare materials · 2026Article
- Ultrasound Modulation of Visual Circuits in Mice Independent of Auditory Confound.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The Role of Piezo 1 in the Study of Intervertebral Disc Degeneration: Phenotype, Mechanism and Treatment.Orthopaedic surgery · 2026Review
- Ultrasonic repression of TRPA1-dependent astrocyte reactivity confers neuroprotection in models of Lewy body dementia.Translational neurodegeneration · 2026Article
- Role of Piezo1 Channels Expressed in PVN in Regulating Sympathetic Nerve Activity and Arterial Blood Pressure in Rats.International journal of molecular sciences · 2026Article
- Precision acoustofluidics for high-throughput mechanobiology in suspension cells.Science advances · 2026Article
- Ultrasound neuromodulation in brain disorders: from mechanotransduction mechanisms to clinical translation.Frontiers in neuroscience · 2026Review
- Piezo1 as a mechanical checkpoint in T cell immunotherapy for solid tumors.Frontiers in pharmacology · 2026Review
- Effects of Low-Intensity Pulsed Ultrasound on the Gut-Brain Axis.Medical devices (Auckland, N.Z.) · 2026Review
- Targeting Piezo1/2 ion channels for modulation of the lymphatic system: mechanisms, applications, and therapeutic prospects.Burns & trauma · 2026Review
- Channel-specific differential effects of bacterial mechanosensitive channels for ultrasound neuromodulation in precision sonogenetics.Theranostics · 2026Article
- Microbubble-enhanced ultrasound stimulation of β-cells improves insulin release and glycemic control in mice.Journal of nanobiotechnology · 2025Article
- Challenges and lessons learned in attempts to develop in vitro models for ultrasound neuromodulation using local field potential measurements in hippocampal tissue.Biophysical journal · 2025Article
- Analysis of the potential regulatory mechanism of PIEZO1 in Alzheimer's disease based on RNA sequencing.IBRO neuroscience reports · 2025Article
- PIEZO2 in tumors: from mechanobiological switches to activity-targeted therapies.Journal of experimental & clinical cancer research : CR · 2025Review
- Alteration of Essential Cell Function by Ultrasound-Targeted Microbubble Cavitation.Current cardiology reports · 2025Review
66 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ultrasound brain stimulation is a promising modality for probing brain function and treating brain disease non-invasively and with high spatiotemporal resolution. However, the mechanism underlying its effects remains unclear. Here, we examine the role that the mouse piezo-type mechanosensitive ion channel component 1 (Piezo1) plays in mediating the in vitro effects of ultrasound in mouse primary cortical neurons and a neuronal cell line. We show that ultrasound alone could activate heterologous and endogenous Piezo1, initiating calcium influx and increased nuclear c-Fos expression in primary neurons but not when pre-treated with a Piezo1 inhibitor. We also found that ultrasound significantly increased the expression of the important proteins phospho-CaMKII, phospho-CREB, and c-Fos in a neuronal cell line, but Piezo1 knockdown significantly reduced this effect. Our findings demonstrate that the activity of mechanosensitive ion channels such as Piezo1 stimulated by ultrasound is an important contributor to its ability to stimulate cells in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.