ArticleJournal of immunological methods2020
Isolation and functional characterisation of lamina propria leukocytes from helminth-infected, murine small intestine.
Article in Journal of immunological methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Mouse CD8+ T cell subsets differentially generate IL-17-expressing cells in the colon epithelium and lamina propria.Clinical and experimental immunology · 2025Article
- Undenatured type II collagen protects against collagen-induced arthritis by restoring gut-joint homeostasis and immunity.Communications biology · 2024Article
- LSD1 drives intestinal epithelial maturation and controls small intestinal immune cell composition independent of microbiota in a murine model.Nature communications · 2024Article
- Metabolic heterogeneity of tissue-resident macrophages in homeostasis and during helminth infection.Nature communications · 2023Article
- l-Ergothioneine Exhibits Protective Effects against Dextran Sulfate Sodium-Induced Colitis in Mice.ACS omega · 2022Article
- Tissue-specific immunity in helminth infections.Mucosal immunology · 2022Review
- Tissue-based IL-10 signalling in helminth infection limits IFNγ expression and promotes the intestinal Th2 response.Mucosal immunology · 2022Article
- Oral delivery of a functional algal-expressed TGF-β mimic halts colitis in a murine DSS model.Journal of biotechnology · 2021Article
- Interrogating the Small Intestine Tuft Cell-ILC2 Circuit Using In Vivo Manipulations.Current protocols · 2021Article
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Authors and funding
5 authors.
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Abstract
The use of helminth infections as tools to understand the type 2 immune response is a well-established technique and important to many areas of immunological research. The phenotype and function of immune cell populations at the site of infection is a key determinant of pathogen clearance. However, infections with helminths such as the murine nematode Heligomosmoides polygryrus cause increased mucus production and thickening of the intestinal wall, which can result in extensive cell death when isolating and analysing cells from the lamina propria (LP). Populations of larger immune cells such as macrophages and dendritic cells are often trapped within mucus or dying tissues. Here we describe an optimised protocol for isolating LP leukocytes from the small intestine of H.polygyrus -infected mice, and we demonstrate phenotypic and functional identification of myeloid and CD4
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