ArticlePlant biotechnology journal2020
Transcription factor BnaA9.WRKY47 contributes to the adaptation of Brassica napus to low boron stress by up-regulating the boric acid channel gene BnaA3.NIP5;1.
Article in Plant biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 78 citations in OpenAlex.
- Transcriptional and post-translational regulation of aquaporins in plants.Stress biology · 2026Review
- MsWRKY49 Modulates Pollen Tube Elongation in Response to Boron Deficiency by Regulating Reactive Oxygen Species Homeostasis in Alfalfa.Plant biotechnology journal · 2026Article
- Effects of exogenous γ-aminobutyric acid concentrations on growth and nutrient uptake in trifoliate orange (Frontiers in plant science · 2026Article
- Review
- Bridging Molecular Insights and Agronomic Innovations: Cutting-Edge Strategies for Overcoming Boron Deficiency in Sustainable Rapeseed Cultivation.Plants (Basel, Switzerland) · 2025Review
- Identification of the bHLH Transcription Factor Family in Orah Mandarin and the Response ofPlants (Basel, Switzerland) · 2025Article
- Transport and regulatory mechanisms of boron in plants.Frontiers in plant science · 2025Review
- Integrative analysis of the transcriptome and proteome reveals the molecular responses of tobacco to boron deficiency.BMC plant biology · 2024Article
- The story of a decade: Genomics, functional genomics, and molecular breeding in Brassica napus.Plant communications · 2024Review
- Physiological and molecular bases of the boron deficiency response in tomatoes.Horticulture research · 2023Article
- Transcriptome Analysis inGenes · 2023Article
- Evolution of theInternational journal of molecular sciences · 2023Article
- Comprehensive analyses of the citrus WRKY gene family involved in the metabolism of fruit sugars and organic acids.Frontiers in plant science · 2023Article
- Multifaceted roles of WRKY transcription factors in abiotic stress and flavonoid biosynthesis.Frontiers in plant science · 2023Review
- Physiological and molecular mechanisms ofFrontiers in plant science · 2023Article
- Targeted genome editing in polyploids: lessons fromFrontiers in plant science · 2023Review
- Genome-Wide Identification of Brassicaceae Hormone-Related Transcription Factors and Their Roles in Stress Adaptation and Plant Height Regulation in Allotetraploid Rapeseed.International journal of molecular sciences · 2022Article
- The application of CRISPR/Cas technologies toaBIOTECH · 2022Review
- Molecular Characterization, Evolutionary Analysis, and Expression Profiling ofPlants (Basel, Switzerland) · 2022Article
- The Xyloglucan Endotransglucosylase/Hydrolase GeneInternational journal of molecular sciences · 2022Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Boron (B) deficiency is one of the major causes of growth inhibition and yield reduction in Brassica napus (B. napus). However, the molecular mechanisms of low B adaptation in B. napus are largely unknown. Here, fifty-one BnaWRKY transcription factors were identified as responsive to B deficiency in B. napus, in which BnaAn.WRKY26, BnaA9.WRKY47, BnaA1.WKRY53 and BnaCn.WRKY57 were tested in yeast one-hybrid assays and showed strong binding activity with conserved sequences containing a W box in the promoters of the B transport-related genes BnaNIP5;1s and BnaBOR1s. Green fluorescent protein fused to the target protein demonstrated the nuclear localization of BnaA9.WRKY47. CRISPR/Cas9-mediated knockout lines of BnaA9.WRKY47 in B. napus had increased sensitivity to low B and lower contents of B than wild-type plants. In contrast, overexpression of BnaA9.WRKY47 enhanced the adaptation to low B with higher B contents in tissues than in wild-type plants. Consistent with the phenotypic response and B accumulation in these transgenic lines, the transcription activity of BnaA3.NIP5;1, a B efficiency candidate gene, was decreased in the knockout lines but was significantly increased in the overexpressing lines under low B conditions. Electrophoretic mobility shift assays, transient expression experiments in tobacco and in situ hybridizations showed that BnaA9.WRKY47 directly activated BnaA3.NIP5;1 expression through binding to the specific cis-element. Taken together, our findings support BnaWRKYs as new participants in response to low B, and BnaA9.WRKY47 contributes to the adaptation of B. napus to B deficiency through up-regulating BnaA3.NIP5;1 expression to facilitate efficient B uptake.
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