ArticleChembiochem : a European journal of chemical biology2020
Rapid Biophysical Characterization and NMR Spectroscopy Structural Analysis of Small Proteins from Bacteria and Archaea.
Article in Chembiochem : a European journal of chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- The Role of Chemistry Across Disciplines From Humanities to Life Sciences in Understanding Complexity and Emergence.Angewandte Chemie (International ed. in English) · 2026Review
- CoMPaseD: advanced planning of proteomic experiments aiming to identify small proteins.microLife · 2026Article
- SProtFP: a machine learning-based method for functional classification of small ORFs in prokaryotes.NAR genomics and bioinformatics · 2025Article
- Guided tour through the small protein landscape ofmicroLife · 2025Article
- C(P)XCG Proteins ofInternational journal of molecular sciences · 2024Article
- A survey of experimental and computational identification of small proteins.Briefings in bioinformatics · 2024Review
- PSPI: A deep learning approach for prokaryotic small protein identification.Frontiers in genetics · 2024Article
- Biochemistry and Protein Interactions of the CYREN Microprotein.Biochemistry · 2023Article
- Exposing the small protein load of bacterial life.FEMS microbiology reviews · 2023Review
- Characterization of the zinc finger μ-protein HVO_0758 fromFrontiers in microbiology · 2023Article
- Revealing the small proteome ofmicroLife · 2023Article
- Mitochondrial polymorphism m.3017C>T of SHLP6 relates to heterothermy.Frontiers in physiology · 2023Article
- Small Proteins; Big Questions.Journal of bacteriology · 2022Article
- Small Proteins in Archaea, a Mainly Unexplored World.Journal of bacteriology · 2022Review
- CdrS Is a Global Transcriptional Regulator Influencing Cell Division in Haloferax volcanii.mBio · 2021Article
- SR7 - a dual-function antisense RNA fromRNA biology · 2021Article
- Article
- Ribosome profiling in archaea reveals leaderless translation, novel translational initiation sites, and ribosome pausing at single codon resolution.Nucleic acids research · 2020Article
- Rapid Biophysical Characterization and NMR Spectroscopy Structural Analysis of Small Proteins from Bacteria and Archaea.Chembiochem : a European journal of chemical biology · 2020Article
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Authors and funding
18 authors at 9 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteins encoded by small open reading frames (sORFs) have a widespread occurrence in diverse microorganisms and can be of high functional importance. However, due to annotation biases and their technically challenging direct detection, these small proteins have been overlooked for a long time and were only recently rediscovered. The currently rapidly growing number of such proteins requires efficient methods to investigate their structure-function relationship. Herein, a method is presented for fast determination of the conformational properties of small proteins. Their small size makes them perfectly amenable for solution-state NMR spectroscopy. NMR spectroscopy can provide detailed information about their conformational states (folded, partially folded, and unstructured). In the context of the priority program on small proteins funded by the German research foundation (SPP2002), 27 small proteins from 9 different bacterial and archaeal organisms have been investigated. It is found that most of these small proteins are unstructured or partially folded. Bioinformatics tools predict that some of these unstructured proteins can potentially fold upon complex formation. A protocol for fast NMR spectroscopy structure elucidation is described for the small proteins that adopt a persistently folded structure by implementation of new NMR technologies, including automated resonance assignment and nonuniform sampling in combination with targeted acquisition.
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