ArticleScientific reports2019
Microarray Embedding/Sectioning for Parallel Analysis of 3D Cell Spheroids.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- High-throughput histopathology for complex in vitro models.Cell reports methods · 2026Article
- An acoustic levitation platform for high-content histological analysis of 3D tissue culture.Lab on a chip · 2025Article
- A 3D Self-Assembly Platform Integrating Decellularized Matrix Recapitulates In Vivo Tumor Phenotypes and Heterogeneity.Cancer research · 2025Article
- PEGDA-based HistoBrick for increasing throughput of cryosectioning and immunohistochemistry in organoid and small tissue studies.Scientific reports · 2025Article
- Engineering Tumor Stroma Morphogenesis Using Dynamic Cell-Matrix Spheroid Assembly.bioRxiv : the preprint server for biology · 2024Article
- Cerebral Organoid Arrays for Batch Phenotypic Analysis in Sections and Three Dimensions.International journal of molecular sciences · 2023Article
- Candidate therapeutic agents in a newly established triple wild-type mucosal melanoma cell line.Cancer communications (London, England) · 2022Article
- Coplanar embedding of multiple 3D cell models in hydrogel towards high-throughput micro-histology.Scientific reports · 2022Article
- Article
- Single cell organization and cell cycle characterization of DNA stained multicellular tumor spheroids.Scientific reports · 2021Article
- The Application of the Tissue Microarray (TMA) Technology to Analyze Cerebral Organoids.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2021Article
- 4D-Printed Transformable Tube Array for High-Throughput 3D Cell Culture and Histology.Advanced materials (Deerfield Beach, Fla.) · 2020Article
- Characterization of Uveal Melanoma Cell Lines and Primary Tumor Samples in 3D Culture.Translational vision science & technology · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three-dimensional cell spheroid models can be used to predict the effect of drugs and therapeutics and to model tissue development and regeneration. The utility of these models is enhanced by high throughput 3D spheroid culture technologies allowing researchers to efficiently culture numerous spheroids under varied experimental conditions. Detailed analysis of high throughput spheroid culture is much less efficient and generally limited to narrow outputs, such as metabolic viability. We describe a microarray approach that makes traditional histological embedding/sectioning/staining feasible for large 3D cell spheroid sample sets. Detailed methodology to apply this technology is provided. Analysis of the technique validates the potential for efficient histological analysis of up to 96 spheroids in parallel. By integrating high throughput 3D spheroid culture technologies with advanced immunohistochemical techniques, this approach will allow researchers to efficiently probe expression of multiple biomarkers with spatial localization within 3D structures. Quantitative comparison of staining will have improved inter- and intra-experimental reproducibility as multiple samples are collectively processed, stained, and imaged on a single slide.
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