ArticleScientific reports2019
A thraustochytrid-specific lipase/phospholipase with unique positional specificity contributes to microbial competition and fatty acid acquisition from the environment.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 32 citations in OpenAlex.
- Structural insights into thraustochytrid-specific lipases using alphafold to identify the role of GXSXG motif.BMC biotechnology · 2025Article
- Transcriptomics aids in uncovering the metabolic shifts and molecular machinery of Schizochytrium limacinum during biotransformation of hydrophobic substrates to docosahexaenoic acid.Microbial cell factories · 2024Article
- The way forward to produce nutraceuticals from agri-food processing residues: obstacle, solution, and possibility.Journal of food science and technology · 2024Review
- The mechanism of Atg15-mediated membrane disruption in autophagy.The Journal of cell biology · 2023Article
- Insights into hydrophobic waste valorization for the production of value-added oleochemicals.Microbial biotechnology · 2023Article
- Viral infection switches the balance between bacterial and eukaryotic recyclers of organic matter during coccolithophore blooms.Nature communications · 2023Article
- Article
- Identification of Extracellular Proteases Induced by Nitrogen-Limited Conditions in the Thraustochytrids Schizochytrium aggregatum ATCC 28209.Marine biotechnology (New York, N.Y.) · 2022Article
- Resolving the Microalgal Gene Landscape at the Strain Level: a Novel Hybrid Transcriptome ofApplied and environmental microbiology · 2022Article
- Degradation of biological macromolecules supports uncultured microbial populations in Guaymas Basin hydrothermal sediments.The ISME journal · 2021Article
- Method Development Progress in Genetic Engineering of Thraustochytrids.Marine drugs · 2021Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thraustochytrids are heterotrophic marine protists that are considered as important decomposers in the marine ecosystem; however, how they digest and uptake lipid nutrients from the environment is largely unknown. Genomic clustering analysis using thraustochytrid draft genome databases revealed that novel proteins with a Lipase_3 domain are commonly present in thraustochytrids, including Aurantiochytrium limacinum. After heterologous expression and His tag-based purification, protein ID: 145138 was identified as lipase/phospholipase capable of hydrolyzing triacylglycerol (TG) and phosphatidylcholine (PC). 145138 was secreted into the medium, and deletion of the 145138 gene in A. limacinum reduced the degradation of extracellular lipids. Fatty acids generated by 145138 were reused for the biosynthesis of PC and TG, and 145138 allowed A. limacinum to survive in the medium containing TG as a sole carbon source. 145138 hydrolyzed all the acyl-ester linkages of TG; however, the enzyme showed strict positional specificity toward phospholipids, generating 2-acyl lysophospholipids. The 2-acyl lysophospholipids showed stronger antimicrobial activity compared with 1-acyl lysophospholipids. These results suggested that 145138 is a bifunctional enzyme that contributes to the acquisition of lipid nutrients from the environment, as well as to generate antimicrobial lysophospholipids that are beneficial for competition with bacteria over lipid nutrients in the marine environment.
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