ArticleBMC plant biology2019
Cloning and functional analysis of the FAD2 gene family from desert shrub Artemisia sphaerocephala.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 26 citations in OpenAlex.
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- Comprehensive Analysis ofInternational journal of molecular sciences · 2025Article
- Genome-wide characterization of FAD gene family in Xanthoceras sorbifolium Bunge and germplasm assessment.PloS one · 2025Article
- The Molecular Cloning and Functional Analysis of thePlants (Basel, Switzerland) · 2024Article
- Crucial amino acids identified in Δ12 fatty acid desaturases related to linoleic acid production inFrontiers in plant science · 2024Article
- Biological Characteristics and Functional Analysis of the Linoleic Acid Synthase GeneInternational journal of molecular sciences · 2023Article
- A mutant cotton fatty acid desaturase 2-1d allele causes protein mistargeting and altered seed oil composition.BMC plant biology · 2023Article
- Integrated Transcriptome and Metabolome Analysis of Rice Leaves Response to High Saline-Alkali Stress.International journal of molecular sciences · 2023Article
- Transcriptome-based analysis of the effects of compound microbial agents on gene expression in wheat roots and leaves under salt stress.Frontiers in plant science · 2023Article
- Insights into the NovelGenes · 2022Article
- Comparative Analysis and Structural Modeling ofBiology · 2022Article
- Functional characterization and expression profile of microsomalPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- TILLING-by-SequencingCells · 2021Article
- Identification, evolution, expression, and docking studies of fatty acid desaturase genes in wheat (Triticum aestivum L.).BMC genomics · 2020Article
- Transformation and Characterization of Δ12-Fatty Acid Acetylenase and Δ12-Oleate Desaturase Potentially Involved in the Polyacetylene Biosynthetic Pathway fromPlants (Basel, Switzerland) · 2020Article
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
Abstract
backgroundLinoleic acid is an important polyunsaturated fatty acid, required for all eukaryotes. Microsomal delta-12 (Δ
resultsFull-length cDNAs of twenty-one AsFAD2 genes were obtained from A. sphaerocephala. The putative polypeptides encoded by AsFAD2 family genes showed a high level of sequence similarity and were relatively conserved during evolution. The motif composition was also relatively conservative. Quantitative real-time PCR analysis revealed that the AsFAD2-1 gene was strongly expressed in developing seeds, which may be closely associated with the high accumulating ability of linoleic acid in A. sphaerocephala seeds. Although different AsFAD2 family members showed diverse response to salt stress, the overall mRNA levels of the AsFAD2 family genes was stable. Transient expression of AsFAD2 genes in the Nicotiana benthamiana leaves revealed that the encoded proteins were all located in the endoplasmic reticulum. Heterologous expression in Saccharomyces cerevisiae suggested that only three AsFAD2 enzymes, AsFAD2-1, - 10, and - 23, were Δ
conclusionsThis research reported the cloning, expression studies, subcellular localization and functional identification of the large AsFAD2 gene family. These results should be helpful in understanding fatty acid biosynthesis in A. sphaerocephala, and has the potential to be applied in the study of plant fatty acids traits.
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