ArticleeLife2019
Non-enzymatic primer extension with strand displacement.
Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed.
- A Facile Strategy for 3'-Terminal Functionalization of DNA and RNA via On-Column Conjugation with Integration into Tandem Oligonucleotide Synthesis.Current protocols · 2026Article
- A Versatile Disulfide-Containing Solid-Support Strategy for 3'-Modifiers in Oligonucleotides: Introducing Modular Tandem Oligonucleotide Synthesis.Chemistry, an Asian journal · 2025Article
- Derivatization of Solid Supports Using a Carbamate-Linked Tether Containing a Disulfide Functional Group.Current protocols · 2025Article
- Trinucleotide substrates under pH-freeze-thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme.Nature chemistry · 2025Article
- Kinetics and dynamics of oligonucleotide hybridization.Nature reviews. Chemistry · 2025Review
- Maximum Entropy Production Principle of Thermodynamics for the Birth and Evolution of Life.Entropy (Basel, Switzerland) · 2025Review
- Simulations predict preferred MgBiophysical journal · 2024Article
- Transient states during the annealing of mismatched and bulged oligonucleotides.Nucleic acids research · 2024Article
- Replication elongates short DNA, reduces sequence bias and develops trimer structure.Nucleic acids research · 2024Article
- Minimal RNA self-reproduction discovered from a random pool of oligomers.Chemical science · 2023Article
- Biological Catalysis and Information Storage Have Relied onBiomolecules · 2023Review
- Experimental Tests of the Virtual Circular Genome Model for Nonenzymatic RNA Replication.Journal of the American Chemical Society · 2023Article
- Ribozyme-mediated RNA synthesis and replication in a model Hadean microenvironment.Nature communications · 2023Article
- DNA Strand Displacement with Base Pair Stabilizers: Purine-2,6-Diamine and 8-Aza-7-Bromo-7-Deazapurine-2,6-Diamine Oligonucleotides Invade Canonical DNA and New Fluorescent Pyrene Click Sensors Monitor the Reaction.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Hybridization kinetics of out-of-equilibrium mixtures of short RNA oligonucleotides.Nucleic acids research · 2022Article
- Rolling Circles as a Means of Encoding Genes in the RNA World.Life (Basel, Switzerland) · 2022Article
- Anomeric DNA Strand Displacement with α-D Oligonucleotides as Invaders and Ethidium Bromide as Fluorescence Sensor for Duplexes with α/β-, β/β- and α/α-D Configuration.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Computer simulations of Template-Directed RNA Synthesis driven by temperature cycling in diverse sequence mixtures.PLoS computational biology · 2022Article
- Thermodynamic and Kinetic Sequence Selection in Enzyme-Free Polymer Self-Assembly inside a Non-equilibrium RNA Reactor.Life (Basel, Switzerland) · 2022Article
- RNA multimerization as an organizing force for liquid-liquid phase separation.RNA (New York, N.Y.) · 2022Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Non-enzymatic RNA self-replication is integral to the emergence of the 'RNA World'. Despite considerable progress in non-enzymatic template copying, demonstrating a full replication cycle remains challenging due to the difficulty of separating the strands of the product duplex. Here, we report a prebiotically plausible approach to strand displacement synthesis in which short 'invader' oligonucleotides unwind an RNA duplex through a toehold/branch migration mechanism, allowing non-enzymatic primer extension on a template that was previously occupied by its complementary strand. Kinetic studies of single-step reactions suggest that following invader binding, branch migration results in a 2:3 partition of the template between open and closed states. Finally, we demonstrate continued primer extension with strand displacement by employing activated 3'-aminonucleotides, a more reactive proxy for ribonucleotides. Our study suggests that complete cycles of non-enzymatic replication of the primordial genetic material may have been facilitated by short RNA oligonucleotides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.