ArticleCommunications biology2019
Enzyme-free release of adhered cells from standard culture dishes using intermittent ultrasonic traveling waves.
Article in Communications biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Article
- Fabrication of a low-adhesion metallic cell culture surface by nanosecond laser processing.Bioprocess and biosystems engineering · 2026Article
- Comparative phenotypic and molecular profiling of replicative and chemically-induced senescence in articular chondrocytes.Cell death discovery · 2026Article
- Dynamic Microstructured Thermoresponsive Interfaces for Label-Free Cell Sorting Based on Nonspecific Interactions.ACS applied materials & interfaces · 2025Article
- Thermoresponsive BrushGel Microcarriers for Efficient Cell Expansion and Enzyme-Reduced Harvesting.Advanced healthcare materials · 2025Article
- Spheroid Cell Aggregation Enhanced by Enzyme-Free Ultrasound-Detached Cells.Advanced biology · 2025Article
- Influence of Exposure Time and Driving Frequency on Cytotoxicity in In Vitro Ultrasound With Constant Mechanical Indices.Engineering in life sciences · 2025Article
- Multi-level analysis of gut microbiome extracellular vesicles-host interaction reveals a connection to gut-brain axis signaling.Microbiology spectrum · 2025Article
- Optical sensor reveals the hidden influence of cell dissociation on adhesion measurements.Scientific reports · 2024Article
- Rebuilding the microenvironment of primary tumors in humans: a focus on stroma.Experimental & molecular medicine · 2024Review
- Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs.Nature communications · 2023Article
- Strategy for Nonenzymatic Harvesting of Cells via Decoupling of Adhesive and Disjoining Domains of Nanostructured Stimulus-Responsive Polymer Films.ACS applied materials & interfaces · 2023Article
- Mechano-responsive hydrogel for direct stem cell manufacturing to therapy.Bioactive materials · 2023Article
- Application of a Magnetic Platform in α6 Integrin-Positive iPSC-TM Purification.Bioengineering (Basel, Switzerland) · 2023Article
- Replacement of Trypsin by Proteases for Medical Applications.Iranian journal of pharmaceutical research : IJPR · 2022Article
- The Progress of Stem Cell Therapy in Myocardial-Infarcted Heart Regeneration: Cell Sheet Technology.Tissue engineering and regenerative medicine · 2022Review
- BPA Decreases PDCD4 in Bovine Granulosa Cells Independently of miR-21 Inhibition.International journal of molecular sciences · 2022Article
- Polydopamine-Mediated Protein Adsorption Alters the Epigenetic Status and Differentiation of Primary Human Adipose-Derived Stem Cells (hASCs).Frontiers in bioengineering and biotechnology · 2022Article
- Article
- An ultrafast enzyme-free acoustic technique for detaching adhered cells in microchannels.RSC advances · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell detachment is essential in culturing adherent cells. Trypsinization is the most popular detachment technique, even though it reduces viability due to the damage to the membrane and extracellular matrix. Avoiding such damage would improve cell culture efficiency. Here we propose an enzyme-free cell detachment method that employs the acoustic pressure, sloshing in serum-free medium from intermittent traveling wave. This method detaches 96.2% of the cells, and increases its transfer yield to 130% of conventional methods for 48 h, compared to the number of cells detached by trypsinization. We show the elimination of trypsinization reduces cell damage, improving the survival of the detached cells. Acoustic pressure applied to the cells and media sloshing from the intermittent traveling wave were identified as the most important factors leading to cell detachment. This proposed method will improve biopharmaceutical production by expediting the amplification of tissue-cultured cells through a more efficient transfer process.
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