Evidence map›Paper›PMID 31700148›Full record

ArticleSpinal cord2020

Gene expression changes are associated with severe bone loss and deficient fracture callus formation in rats with complete spinal cord injury.

Mariana M Butezloff, José B Volpon, João P B Ximenez, Kelly Astolpho, Vitor M Correlo, Rui L Reis, Raquel B Silva, Ariane Zamarioli

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Spinal cord, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it, 8 citations in OpenAlex.

  1. Beyond the initial impact: a systematic review of post-traumatic bone loss and its mechanisms.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2026
    Pooled it
  2. Article
  3. Article
  4. Article
  5. The Pathophysiology of Osteoporosis after Spinal Cord Injury.International journal of molecular sciences · 2021
    Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Mariana M ButezloffSchool of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
José B VolponSchool of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
João P B XimenezSchool of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
Kelly AstolphoSchool of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
Vitor M Correlo3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, Braga, Portugal.
Rui L Reis3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, Braga, Portugal.
Raquel B SilvaSchool of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
Ariane ZamarioliSchool of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil. arianezamarioli@usp.br.ORCID http://orcid.org/0000-0002-7939-0528
Universidade de São Paulo · BRUniversity of Minho · PTUniversidade de Ribeirão Preto · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

STUDY

designAnimal study.

objectivesTo investigate the effects of SCI on bone quality and callus formation.

settingUniversity and hospital-based research center, Ribeirão Preto Medical School, Brazil.

methodsRats sustaining a complete SCI for 10 days received a fracture at the femoral diaphysis and were followed-up for 14 days. Bone callus and contralateral nonfractured tibia were assessed by DXA, µCT, ELISA, histomorphometry, immunohistochemistry, biomechanical test, and gene expression.

resultsSCI downregulated osteoblastic-related gene expression in the nonfractured tibias, associated with a twofold increase in osteoclasts and overexpression of RANK/RANKL, which resulted in lower bone mass, impaired microarchitecture, and weaker bones. On day 14 postfracture, we revealed early and increased trabecular formation in the callus of SCI rats, despite a marked 75% decrease in OPG-positive cells, and 41% decrease in density. Furthermore, these calluses showed higher porosity and thinner newly formed trabeculae, leading to lower strength and angle failure.

conclusionsSCI-induced bone loss resulted from increased bone resorption and decreased bone formation. We also evidenced accelerated bone healing in the SCI rats, which may be attributed to the predominant intramembranous ossification. However, the newly formed bone was thinner, less dense, and more porous than those in the non-SCI rats. As a result, these calluses are weaker and tolerate lesser torsion deformation than the controls, which may result in recurrent fractures and characterizes a remarkable feature that may severely impair life quality.

Indexed as

AnimalsBone ResorptionBony CallusCancellous BoneDisease Models, AnimalDown-RegulationFemurFractures, BoneGene ExpressionMaleOsteoblastsOsteogenesisRatsRats, WistarSpinal Cord InjuriesTibia

Identifiers

PMID31700148
OpenAlexW2985064589

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.