ArticleScientific reports2019
A molecular clone of Chronic Bee Paralysis Virus (CBPV) causes mortality in honey bee pupae (Apis mellifera).
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 19 citations in OpenAlex.
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- A DNA Plasmid-Based Approach for Efficient Synthesis of Sacbrood Virus Infectious Clones within Host Cells.Viruses · 2023Article
- Essential Oils for a Sustainable Control of Honeybee Varroosis.Veterinary sciences · 2023Review
- Prevalence of honey bee pathogens and parasites in South Korea: A five-year surveillance study from 2017 to 2021.Heliyon · 2023Article
- Crude Extracts ofViruses · 2023Article
- A derived honey bee stock confers resistance to Varroa destructor and associated viral transmission.Scientific reports · 2022Article
- Procedures and potential pitfalls for constructing a bee-infecting RNA virus clone.Frontiers in insect science · 2022Review
- Organization of the Structural Protein Region of La Jolla Virus Isolated from the Invasive Pest InsectViruses · 2021Article
- Chemical Stimulants and Stressors Impact the Outcome of Virus Infection and Immune Gene Expression in Honey Bees (Frontiers in immunology · 2021Article
- The Honey Bee GeneFrontiers in insect science · 2021Article
- A Reverse Genetics System for the Israeli Acute Paralysis Virus and Chronic Bee Paralysis Virus.International journal of molecular sciences · 2020Article
- Visualizing Sacbrood Virus of Honey Bees via Transformation and Coupling with Enhanced Green Fluorescent Protein.Viruses · 2020Article
- Bee Viruses: Routes of Infection in Hymenoptera.Frontiers in microbiology · 2020Review
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Authors and funding
7 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Among the many diseases compromising the well-being of the honey bee (Apis mellifera) the chronic paralysis syndrome of adult honey bees is one of the best described. The causative agent, chronic bee paralysis virus (CBPV), is a positive sense, single-stranded RNA virus with a segmented genome. Segment 1 encodes three putative open reading frames (ORFs), including the RNA-dependent RNA polymerase and other non-structural protein coding regions. Segment 2 encodes four putative ORFs, which contain the genes of supposed structural proteins. In this study, we established a reverse genetic system for CBPV by molecular cloning of DNA copies of both genome segments. CBPV rescue was studied in imago and honey bee pupae infection models. Virus replication and progeny virus production was only initiated when capped RNAs of both genome segments were injected in honey bees. As injection of these clonal RNAs caused clinical symptoms similar to wild-type CBPV infection, we conclude that the novel molecular clone fulfilled Koch's postulates. Our virus clone will enable in-depth analysis of CBPV pathogenesis and help to increase knowledge about this important honey bee disease.
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