ArticleMicrobial cell factories2019
High production of valencene in Saccharomyces cerevisiae through metabolic engineering.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 67 citations in OpenAlex.
- Highly efficient production ofSynthetic and systems biotechnology · 2027Article
- RNA Polymerase III Promoters Compatible with CRISPR Gene Regulation inACS synthetic biology · 2025Article
- Highly efficient synthesis of the natural product β-himachalene in yeast and its potential as a novel anti-HSV-1 therapeutic agent.Synthetic and systems biotechnology · 2025Article
- Characterization and functional reconstruction of a highly productive germacrene A synthase from Liriodendron chinense.Plant biotechnology journal · 2025Article
- Development of a Komagataella phaffii cell factory for sustainable production of ( +)-valencene.Microbial cell factories · 2025Article
- New insights into the roles of fungi and bacteria in the development of medicinal plant.Journal of advanced research · 2024Review
- Article
- Application of valencene and prospects for its production in engineered microorganisms.Frontiers in microbiology · 2024Review
- Advances in the dynamic control of metabolic pathways inEngineering microbiology · 2023Review
- An outlook to sophisticated technologies and novel developments for metabolic regulation in theFrontiers in bioengineering and biotechnology · 2023Review
- Prediction of strain engineerings that amplify recombinant protein secretion through the machine learning approach MaLPHAS.Engineering biology · 2022Article
- High-yield production of protopanaxadiol from sugarcane molasses by metabolically engineered Saccharomyces cerevisiae.Microbial cell factories · 2022Article
- Revolution of vitamin E production by starting from microbial fermented farnesene to isophytol.Innovation (Cambridge (Mass.)) · 2022Article
- Metabolic engineering ofMetabolic engineering communications · 2021Article
- Heterologous Production of β-Caryophyllene and Evaluation of Its Activity against Plant Pathogenic Fungi.Microorganisms · 2021Article
- CRISPR-Cas9: A Powerful Tool to Efficiently EngineerLife (Basel, Switzerland) · 2020Review
- Harnessing sub-organelle metabolism for biosynthesis of isoprenoids in yeast.Synthetic and systems biotechnology · 2020Review
- Review
- Article
- Microbial Cell Factory for Efficiently Synthesizing Plant Natural Products via Optimizing the Location and Adaptation of Pathway on Genome Scale.Frontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe biological synthesis of high value compounds in industry through metabolically engineered microorganism factories has received increasing attention in recent years. Valencene is a high value ingredient in the flavor and fragrance industry, but the low concentration in nature and high cost of extraction limits its application. Saccharomyces cerevisiae, generally recognized as safe, is one of the most commonly used gene expression hosts. Construction of S. cerevisiae cell factory to achieve high production of valencene will be attractive.
resultsValencene was successfully biosynthesized after introducing valencene synthase into S. cerevisiae BJ5464. A significant increase in valencene yield was observed after down-regulation or knock-out of squalene synthesis and other inhibiting factors (such as erg9, rox1) in mevalonate (MVA) pathway using a recyclable CRISPR/Cas9 system constructed in this study through the introduction of Cre/loxP. To increase the supplement of the precursor farnesyl pyrophosphate (FPP), all the genes of FPP upstream in MVA pathway were overexpressed in yeast genome. Furthermore, valencene expression cassettes containing different promoters and terminators were compared, and P
conclusionsThis study achieved high production of valencene in S. cerevisiae through metabolic engineering and optimization of expression cassette, providing good example of microbial overproduction of valuable chemical products. The construction of recyclable plasmid was useful for multiple gene editing as well.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.