ArticleBMC biotechnology2019
Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection.
Article in BMC biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 23 citations in OpenAlex.
- Simple, fast, reliable: multiplex digital PCR quantification of 19 genetically modified soybean events.GM crops & food · 2026Article
- Multiplex PCR Fluorescence Method for Detection of Genetically Modified Maize Strains.Current issues in molecular biology · 2026Article
- Advanced Nanomaterial-Based Electrochemical Biosensing of Loop-Mediated Isothermal Amplification Products.Biosensors · 2025Article
- Future perspectives of GMO detection in agriculture: strategies for electrochemical nucleic acid detection.Mikrochimica acta · 2025Review
- Development of a novel Cas13a/Cas12a-mediated 'one-pot' dual detection assay for genetically modified crops.Journal of advanced research · 2025Article
- Multiplex PCR assay for the simultaneous identification of race specific and non-specific leaf resistance genes in wheat (Triticum aestivum L.).Journal of applied genetics · 2023Article
- Qualitative and Quantitative Real-Time PCR Methods for Assessing False-Positive Rates in Genetically Modified Organisms Based on the Microbial-Infection-LinkedInternational journal of molecular sciences · 2022Article
- Detection and identification of authorized and unauthorized GMOs using high-throughput sequencing with the support of a sequence-based GMO database.Food chemistry. Molecular sciences · 2022Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMost genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assays designed to detect GM plants often target the P35S and/or TNOS DNA sequences. However, because the P35S promoter is derived from CaMV, these detection assays can yield false-positives from non-GM plants infected by this naturally-occurring virus.
resultsHere we report the development of an assay designed to distinguish CaMV-infected plants from GM plants in a single multiplexed quantitative PCR (qPCR) reaction. Following initial testing and optimization via PCR and singleplex-to-multiplex qPCR on both plasmid and plant DNA, TaqMan qPCR probes with different fluorescence wavelengths were designed to target actin (a positive-control plant gene), P35S, P3 (a CaMV-specific gene), and TNOS. We tested the specificity of our quadruplex qPCR assay using different DNA extracts from organic watercress and both organic and GM canola, all with and without CaMV infection, and by using commercial and industrial samples. The limit of detection (LOD) of each target was determined to be 1% for actin, 0.001% for P35S, and 0.01% for both P3 and TNOS.
conclusionsThis assay was able to distinguish CaMV-infected plants from GM plants in a single multiplexed qPCR reaction for all samples tested in this study, suggesting that this protocol is broadly applicable and readily transferrable to any interested parties with a qPCR platform.
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