Evidence map›Paper›PMID 31697956›Full record

ArticleDevelopmental and comparative immunology2020

Duck karyopherin α4 (duKPNA4) is involved in type I interferon expression and the antiviral response against Japanese encephalitis virus.

Chenxi Li, Di Di, Xin Wang, Qiqi Xia, Abdul Wahaab, Muhammad Naveed Anwar, Zongjie Li, Ke Liu, Donghua Shao, Yafeng Qiu and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental and comparative immunology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Antiviral Effects of Avian Interferon-Stimulated Genes.Animals : an open access journal from MDPI · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Chenxi LiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No.518, Ziyue Road, Shanghai, 200241, PR China.
Di DiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No.518, Ziyue Road, Shanghai, 200241, PR China.
Xin WangShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No.518, Ziyue Road, Shanghai, 200241, PR China.
Qiqi XiaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No.518, Ziyue Road, Shanghai, 200241, PR China.
Abdul WahaabShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No.518, Ziyue Road, Shanghai, 200241, PR China.
Muhammad Naveed AnwarShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No.518, Ziyue Road, Shanghai, 200241, PR China.
Zongjie LiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No.518, Ziyue Road, Shanghai, 200241, PR China.
Ke LiuShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No.518, Ziyue Road, Shanghai, 200241, PR China.
Donghua ShaoShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No.518, Ziyue Road, Shanghai, 200241, PR China.
Yafeng QiuShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No.518, Ziyue Road, Shanghai, 200241, PR China.
Jianchao WeiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No.518, Ziyue Road, Shanghai, 200241, PR China.
Beibei LiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No.518, Ziyue Road, Shanghai, 200241, PR China. Electronic address: lbb@shvri.ac.cn.
Zhiyong MaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No.518, Ziyue Road, Shanghai, 200241, PR China. Electronic address: zhiyongma@shvri.ac.cn.
Shanghai Veterinary Research Institute · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Karyopherin α4 (KPNA4) is an adaptor molecule that mediates type I interferon (IFN) production by facilitating the nuclear translocation of IFN transcription factors. Here, we cloned the duck KPNA4 (duKPNA4) gene and analyzed its involvement in type I IFN expression as well as antiviral response against Japanese encephalitis virus (JEV). The full-length duKPNA4 gene encoded a 520-amino acid protein that shared 97.3-98.7% sequence similarity with its orthologues in chickens, humans and mice. The duKPNA4 was extensively expressed in various duck tissues at the mRNA level. Analysis of the subcellular localization of duKPNA4 by immunofluorescence assays indicated that the duKPNA4 was primarily distributed in both the cytoplasm and nucleus in primary duck embryonic fibroblasts (DEFs). However, it translocated from the cytoplasm to the nucleus in response to poly(I:C) stimulation or JEV infection. The duKPNA4 interacted with duck IFN regulatory factor 7 and facilitated its nuclear translocation, thereby up-regulating the expression of IFN-α and IFN-β in DEFs in the presence of poly(I:C) stimulation. Exogenous expression of duKPNA4 significantly elevated the expression of IFN-α and IFN-β induced by JEV infection and inhibited JEV replication in DEFs. These data demonstrate the importance of duKPNA4 in type I IFN signaling as well as the antiviral response against JEV replication.

Indexed as

alpha KaryopherinsAnimalsAvian ProteinsCells, CulturedCloning, MolecularDucksEncephalitisEncephalitis Virus, JapaneseFibroblastsImmunityInterferon Regulatory Factor-7Interferon Type IProtein TransportSignal TransductionVirus Replicationalpha KaryopherinsAvian ProteinsInterferon Regulatory Factor-7Interferon Type IAntiviral responseDuck karyopherin α4Interferon transcription factor 7Japanese encephalitis virusType I interferon

Identifiers

PMID31697956
PMCPMC7102526
OpenAlexW2989375994

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.