ArticleTissue engineering. Part A2020
Multimaterial Dual Gradient Three-Dimensional Printing for Osteogenic Differentiation and Spatial Segregation.
Article in Tissue engineering. Part A, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Combining Fluid Dynamics and Biological Experiments to Explore the More Suitable Porosity for Bone Inducing Porous Tantalum Scaffolds.Orthopaedic surgery · 2026Article
- Organoid bioprinting to pattern the matrix microenvironment.Current opinion in biomedical engineering · 2025Article
- Gradient scaffolds in bone-soft tissue interface engineering: Structural characteristics, fabrication techniques, and emerging trends.Journal of orthopaedic translation · 2025Review
- Advances in Additive Manufactured Scaffolds Mimicking the Osteochondral Interface.Advanced nanobiomed research · 2024Article
- Controlled Mechanical Property Gradients Within a Digital Light Processing Printed Hydrogel-Composite Osteochondral Scaffold.Annals of biomedical engineering · 2024Article
- Morphological Integrated Preparation Method and Implementation of Inorganic/Organic Dual-Phase Composite Gradient Bionic Bone Scaffold.3D printing and additive manufacturing · 2024Article
- Diffusion-Based 3D Bioprinting Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- The effect of multi-material architecture on the ex vivo osteochondral integration of bioprinted constructs.Acta biomaterialia · 2023Article
- Development of photoreactive demineralized bone matrix 3D printing colloidal inks for bone tissue engineering.Regenerative biomaterials · 2023Article
- Installation of click-type functional groups enable the creation of an additive manufactured construct for the osteochondral interface.Biofabrication · 2022Article
- Bioprinted Membranes for Corneal Tissue Engineering: A Review.Pharmaceutics · 2022Review
- Esophagus tissue engineering: from decellularization to in vivo recellularization in two sites.Cell and tissue banking · 2022Article
- Article
- Effect of 3D Printing Temperature on Bioactivity of Bone Morphogenetic Protein-2 Released from Polymeric Constructs.Annals of biomedical engineering · 2021Article
- Swelling Behaviors of 3D Printed Hydrogel and Hydrogel-Microcarrier Composite Scaffolds.Tissue engineering. Part A · 2021Article
- Development of a modular, biocompatible thiolated gelatin microparticle platform for drug delivery and tissue engineering applications.Regenerative biomaterials · 2021Article
- Three-Dimensional Printing of Click Functionalized, Peptide Patterned Scaffolds for Osteochondral Tissue Engineering.Bioprinting (Amsterdam, Netherlands) · 2021Article
- Cryogenic 3D Printing ofFrontiers in bioengineering and biotechnology · 2021Article
- Fiber engraving for bioink bioprinting within 3D printed tissue engineering scaffolds.Bioprinting (Amsterdam, Netherlands) · 2020Article
- Three-Dimensional Extrusion Printing of Porous Scaffolds Using Storable Ceramic Inks.Tissue engineering. Part C, Methods · 2020Article
Corrections and comments
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Authors and funding
15 authors at 5 institutions in 1 country.
Funding
Abstract
In this study of three-dimensional (3D) printed composite β-tricalcium phosphate (β-TCP)-/hydroxyapatite/poly(ɛ-caprolactone)-based constructs, the effects of vertical compositional ceramic gradients and architectural porosity gradients on the osteogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells (MSCs) were investigated. Specifically, three different concentrations of β-TCP (0, 10, and 20 wt%) and three different porosities (33% ± 4%, 50% ± 4%, and 65% ± 3%) were examined to elucidate the contributions of chemical and physical gradients on the biochemical behavior of MSCs and the mineralized matrix production within a 3D culture system. By delaminating the constructs at the gradient transition point, the spatial separation of cellular phenotypes could be specifically evaluated for each construct section. Results indicated that increased concentrations of β-TCP resulted in upregulation of osteogenic markers, including alkaline phosphatase activity and mineralized matrix development. Furthermore, MSCs located within regions of higher porosity displayed a more mature osteogenic phenotype compared to MSCs in lower porosity regions. These results demonstrate that 3D printing can be leveraged to create multiphasic gradient constructs to precisely direct the development and function of MSCs, leading to a phenotypic gradient. Impact Statement In this study, three-dimensional (3D) printed ceramic/polymeric constructs containing discrete vertical gradients of both composition and porosity were fabricated to precisely control the osteogenic differentiation of mesenchymal stem cells. By making simple alterations in construct architecture and composition, constructs containing heterogenous populations of cells were generated, where gradients in scaffold design led to corresponding gradients in cellular phenotype. The study demonstrates that 3D printed multiphasic composite constructs can be leveraged to create complex heterogeneous tissues and interfaces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.