ArticleEuropean journal of histochemistry : EJH2019
Bovine pericardium membrane, gingival stem cells, and ascorbic acid: a novel team in regenerative medicine.
Article in European journal of histochemistry : EJH, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Effects of TNF-Journal of clinical medicine · 2026Article
- Exploring the bioactive potential of bovine pericardium membrane combined with hyaluronic Acid: characterization and cellular viability analyses.Biochemistry and biophysics reports · 2026Article
- Post-translational modifications in osteogenic differentiation of oral-derived stem cells: Mechanisms and clinical implications.World journal of stem cells · 2025Review
- Ultrastructural assessment of human periodontal ligament fibroblast interaction with bovine pericardium membranes: AnHistology and histopathology · 2025Article
- Zinc nanoparticles induced eggshell collagen membrane used for guided bone regeneration: A novel approach in rabbit models.Odontology · 2025Article
- Geometrically modified bovine pericardium membrane promotes the expression of molecules targeted for a faster integration and vascularization process.Frontiers in bioengineering and biotechnology · 2024Article
- Inhibition of LPS-Induced Inflammatory Response of Oral Mesenchymal Stem Cells in the Presence of Galectin-3.Biomedicines · 2023Article
- Guide bone regeneration (GBR) reconstruction of extensive alveolar bone defect due to infected radicular cyst enucleation: 2 case reports.International journal of surgery case reports · 2023Article
- Proliferation and Morphological Assessment of Human Periodontal Ligament Fibroblast towards Bovine Pericardium Membranes: An In Vitro Study.Materials (Basel, Switzerland) · 2022Article
- A Narrative Review: Gingival Stem Cells as a Limitless Reservoir for Regenerative Medicine.International journal of molecular sciences · 2022Review
- The Application of a Bone Marrow Mesenchymal Stem Cell Membrane in the Vascularization of a Decellularized Tracheal Scaffold.Stem cells international · 2021Article
- Fabrication and Characterization of Collagen/PVA Dual-Layer Membranes for Periodontal Bone Regeneration.Frontiers in bioengineering and biotechnology · 2021Article
- Gingiva-Derived Mesenchymal Stem Cells: Potential Application in Tissue Engineering and Regenerative Medicine - A Comprehensive Review.Frontiers in immunology · 2021Review
- Twenty years of histochemistry in the third millennium, browsing the scientific literature.European journal of histochemistry : EJH · 2020Article
- A journal of histochemistry as a forum for non-histochemical scientific societies.European journal of histochemistry : EJH · 2019Article
- Gingival mesenchymal stem cells: Biological properties and therapeutic applications.Journal of oral biology and craniofacial researchArticle
- Retinoic and ascorbic acids induce osteoblast differentiation from human dental pulp mesenchymal stem cells.Journal of oral biology and craniofacial researchArticle
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, the development and the application of 3D scaffold able to promote stem cell differentiation represented an essential field of interest in regenerative medicine. In particular, functionalized scaffolds improve bone tissue formation and promote bone defects repair. This research aims to evaluate the role of ascorbic acid (AS) supplementation in an in vitro model, in which a novel 3D-scaffold, bovine pericardium collagen membrane called BioRipar (BioR) was functionalized with human Gingival Mesenchymal Stem Cells (hGMSCs). As extensively reported in the literature, AS is an essential antioxidant molecule involved in the extracellular matrix secretion and in the osteogenic induction. Specifically, hGMSCs were seeded on BioR and treated with 60 and 90 μg/mL of AS in order to assess their growth behavior, the expression of bone specific markers involved in osteogenesis (runt-related transcription factor 2, RUNX2; collagen1A1, COL1A1; osteopontin, OPN; bone morphogenetic protein2/4, BMP2/4), and de novo deposition of calcium. The expression of COL1A1, RUNX2, BMP2/4 and OPN was evaluated by RT-PCR, Western blotting and immunocytochemistry, and proved to be upregulated. Our results demonstrate that after three weeks of treatment AS at 60 and 90 μg/mL operates as an osteogenic inductor in hGMSCs. These data indicate that the AS supplementation produces an enhancement of osteogenic phenotype commitment in an in vitro environment. For this reason, AS could represent a valid support for basic and translational research in tissue engineering and regenerative medicine.
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Registered trials
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