Evidence map›Paper›PMID 31696039›Full record

Article3 Biotech2019

Deciphering the transcriptomic insight during organogenesis in Castor (

Sai Sudha Puvvala, Tarakeswari Muddanuru, Padmavathi A V Thangella, O Aniel Kumar, Navajeet Chakravartty, Vineeth Kodengil Vettath, A V S K Mohan Katta, Sivarama Prasad Lekkala, Boney Kuriakose, Saurabh Gupta and 2 more

Open access · greenAbstract read
In one paragraph

Article in 3 Biotech, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Sai Sudha Puvvala *1Andhra University, Visakhapatnam, 530 003 India.
Tarakeswari Muddanuru *2ICAR-Indian Institute of Oilseeds Research (Formerly DOR), Rajendranagar, Hyderabad, 500 007 India.
Padmavathi A V Thangella3Osmania University, Tarnaka, Hyderabad, 500 007 India.
O Aniel Kumar1Andhra University, Visakhapatnam, 530 003 India.
Navajeet ChakravarttyAgriGenome Labs Pvt. Ltd, BTIC, MN iHub, Genome Valley, Hyderabad, Telangana State 500 078 India.
Vineeth Kodengil VettathAgriGenome Labs Pvt. Ltd, SmartCity Kochi, Infopark Road, Kakkanad, 682 042 India.
A V S K Mohan KattaAgriGenome Labs Pvt. Ltd, BTIC, MN iHub, Genome Valley, Hyderabad, Telangana State 500 078 India.
Sivarama Prasad LekkalaAgriGenome Labs Pvt. Ltd, BTIC, MN iHub, Genome Valley, Hyderabad, Telangana State 500 078 India.
Boney KuriakoseAgriGenome Labs Pvt. Ltd, SmartCity Kochi, Infopark Road, Kakkanad, 682 042 India.
Saurabh GuptaAgriGenome Labs Pvt. Ltd, BTIC, MN iHub, Genome Valley, Hyderabad, Telangana State 500 078 India.ORCID 0000-0002-1327-7389
Sujatha Mulpuri2ICAR-Indian Institute of Oilseeds Research (Formerly DOR), Rajendranagar, Hyderabad, 500 007 India.
Vijaya Bhaskar Reddy LachagariAgriGenome Labs Pvt. Ltd, BTIC, MN iHub, Genome Valley, Hyderabad, Telangana State 500 078 India.
SciGenom Labs (India) · INAndhra University · INIndian Institute of Oilseeds Research · INOsmania University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cultivation of the castor crop is hindered by various factors and one of the approaches for genetic improvement of the crop is through exploitation of biotechnological tools. Response of castor tissues to in vitro culture is poor which necessitated this study on understanding the molecular basis of organogenesis in cultured tissues of castor, through de novo transcriptome analysis and by comparing with jatropha and sunflower having good regeneration ability. Transcriptome profiling analysis was carried out with hypocotyl explants from castor, jatropha and cotyledons from sunflower cultured on MS media supplemented with different concentrations of hormones. Differentially expressed genes during dedifferentiation and organogenic differentiation stages of callus included components of auxin and cytokinin signaling, secondary metabolite synthesis, genes encoding transcription factors, receptor kinases and protein kinases. In castor, many genes involved in auxin biosynthesis and homeostasis like WAT1, vacuolar transporter genes, transcription factors like short root like protein were down-regulated while genes like DELLA were up-regulated accounting for regeneration recalcitrance. Validation of 62 DEGs through qRT-PCR showed a consensus of 77.4% of the genes expressed. Overall study provides set of genes involved in the process of organogenesis in three oilseed crops which forms a basis for understanding and improving the efficiency of plant regeneration and genetic transformation in castor.

Indexed as

AuxinsCallusCastorDEGsOrganogenesisRNA-seq

Identifiers

PMID31696039
PMCPMC6825063
OpenAlexW2988772719

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.