ArticleScientific reports2019
Expansion, isolation and first characterization of bovine Th17 lymphocytes.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Antiviral and anti-inflammatory evaluation of herbal extracts: Implications for the management of calf diarrheal diseases.PloS one · 2026Article
- Calcium Release-Activated Calcium Modulator ORAI1-Sensitive Serine Dehydratase Regulates Fatty Acid-Induced CD4Animals : an open access journal from MDPI · 2025Article
- Evaluating the inhibition of IL-17A and TNFα in a cartilage explant model cultured with Th17-derived cytokines.Journal of translational autoimmunity · 2024Article
- Staphylococcal mastitis in dairy cows.Frontiers in veterinary science · 2024Review
- Punch-excised explants of bovine mammary gland to model early immune response to infection.Journal of animal science and biotechnology · 2023Article
- Microbiota-host crosstalk in the newborn and adult rumen at single-cell resolution.BMC biology · 2022Article
- Delineating transcriptional crosstalk between Mycobacterium avium subsp. paratuberculosis and human THP-1 cells at the early stage of infection via dual RNA-seq analysis.Veterinary research · 2022Article
- A time-resolved multi-omics atlas of Acanthamoeba castellanii encystment.Nature communications · 2022Article
- Progress towards the Elusive Mastitis Vaccines.Vaccines · 2022Review
- Simplified Approaches for the Production of Monocyte-Derived Dendritic Cells and Study of Antigen Presentation in Bovine.Frontiers in veterinary science · 2022Article
- The Effects of Ursolic Acid Treatment on Immunopathogenesis FollowingFrontiers in veterinary science · 2021Article
- Bovine Immunology: Implications for Dairy Cattle.Frontiers in immunology · 2021Review
- Revealing immune responses in the Mycobacterium avium subsp. paratuberculosis-infected THP-1 cells using single cell RNA-sequencing.PloS one · 2021Article
- Epithelial processed Mycobacterium avium subsp. paratuberculosis induced prolonged Th17 response and suppression of phagocytic maturation in bovine peripheral blood mononuclear cells.Scientific reports · 2020Article
- Type 3 immunity: a perspective for the defense of the mammary gland against infections.Veterinary research · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interleukin 17A-producing T helper cells (Th17) are CD4+ T cells that are crucial to immunity to extracellular bacteria. The roles of these cells in the bovine species are poorly defined, because the characterization of bovine Th17 cells lags behind for want of straightforward cultivation and isolation procedures. We have developed procedures to differentiate, expand, and isolate bovine Th17 cells from circulating CD4+ T cells of adult cows. Using polyclonal stimulation with antibodies to CD3 and CD28, we expanded IL-17A-positive CD4+ T cells in a serum-free cell culture medium supplemented with TGF-β1, IL-6 and IL-2. Populations of CD4+ T cells producing IL-17A or IFN-γ or both cytokines were obtained. Isolation of IL-17A-secreting CD4+ T cells was performed by labelling surface IL-17A, followed by flow cytometry cell sorting. The sorted Th17 cells were restimulated and could be expanded for several weeks. These cells were further characterized by cytokine profiling at transcriptomic and protein levels. They produced high amounts of IL-17A and IL-17F, and moderate amounts of IL-22 and IFN-γ. The techniques developed will be useful to characterize the phenotypic and functional properties of bovine Th17 cells.
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