Evidence map›Paper›PMID 31694918›Full record

ArticleThe Journal of biological chemistry2019

Facile autofluorescence suppression enabling tracking of single viruses in live cells.

Yen-Cheng Chen, Chetan Sood, Ashwanth C Francis, Gregory B Melikyan, Robert M Dickson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yen-Cheng ChenSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332-0400.
Chetan SoodDepartment of Pediatrics, Emory School of Medicine, Atlanta, Georgia 30322.
Ashwanth C FrancisDepartment of Pediatrics, Emory School of Medicine, Atlanta, Georgia 30322.ORCID 0000-0002-8663-2038
Gregory B MelikyanDepartment of Pediatrics, Emory School of Medicine, Atlanta, Georgia 30322 gmeliki@emory.edu.
Robert M DicksonSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332-0400 dickson@chemistry.gatech.edu.

Funding

Imaging of Single HIV-1 Uncoating and Transport to the nucleusR01AI129862 · NIAID · EMORY UNIVERSITY · PI KVARATSKHELIA, MAMUKA, MELIKIAN, GREGORY B · 2017 to 2025
$5.4M
Biophysics of Protein-Mediated Membrane FusionR37AI150453 · NIAID · EMORY UNIVERSITY · PI Gregory B Melikian · 2020 to 2026
$4.1M
Biophysics of Protein-Mediated Membrane FusionR01AI150453 · NIAID · EMORY UNIVERSITY · PI MELIKIAN, GREGORY B · 2019 to 2019
$466k
Expanded dimensionality and high sensitivity cell imaging using designed OMFPsR21EB020371 · NIBIB · GEORGIA INSTITUTE OF TECHNOLOGY · PI DICKSON, ROBERT M · 2015 to 2016
$401k
NIAID NIH HHS R01 AI129862NIAID NIH HHS R01 AI150453NIAID NIH HHS R37 AI150453NIBIB NIH HHS R21 EB020371
6 · The paper itself

Abstract

Live cell fluorescence imaging is the method of choice for studying dynamic processes, such as nuclear transport, vesicular trafficking, and virus entry and egress. However, endogenous cellular autofluorescence masks a useful fluorescence signal, limiting the ability to reliably visualize low-abundance fluorescent proteins. Here, we employed synchronously amplified fluorescence image recovery (SAFIRe), which optically alters ground

Indexed as

FluorescenceOptical ImagingAnimalsCell SurvivalGreen Fluorescent ProteinsHEK293 CellsHIV-1HumansMiceMicroscopy, FluorescenceNIH 3T3 Cellsenhanced green fluorescent proteinGreen Fluorescent Proteinsautofluorescencehuman immunodeficiency virus (HIV)membrane fusionmicrobiologymicroscopic imagingoptical modulationphotoswitchable fluorescent proteinssingle virus trackingvirus entry

Identifiers

PMID31694918
PMCPMC6916469

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.