ArticleParasites & vectors2019
High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis.
Article in Parasites & vectors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- Hemotropic mono- and coinfections in Colombian ruminants: descriptive occurrence and host-related factors associated with coinfection in cattle.Tropical animal health and production · 2026Article
- Advances and Challenges in the Diagnosis of Vector-Borne Protozoal Infections in Veterinary Medicine.Pathogens (Basel, Switzerland) · 2026Review
- A One-Pot RPA-CRISPR/Cas12a Assay for Rapid Genus-Level Detection of Babesia spp. in Ticks and Livestock Blood Samples.Transboundary and emerging diseases · 2026Article
- Assessing Different PCR Master Mixes for Ultrarapid DNA Amplification: Important Analytical Parameters.Diagnostics (Basel, Switzerland) · 2024Article
- A high-resolution melting approach for the simultaneous differentiation of five human babesiosis-causing Babesia species.Parasites & vectors · 2023Article
- High-resolution melting analysis identifies reservoir hosts of zoonotic Leishmania parasites in Tunisia.Parasites & vectors · 2022Article
- Cross-sectional survey of cattle haemopathogens in Constantine, Northeast Algeria.Veterinary medicine and science · 2021Article
- Knowledge, attitude and perception of bovine piroplasmosis by cattle owners in Constantine, North-East of Algeria, using participatory epidemiology.Tropical animal health and production · 2021Article
- Systematic Review of Ticks and Tick-Borne Pathogens of Small Ruminants in Pakistan.Pathogens (Basel, Switzerland) · 2020Review
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundBovine babesiosis is caused by protozoan parasites of the genus Babesia and presents a wide spectrum of clinical manifestations. Disease severity depends on the type of Babesia species infection. Generally, B. bovis and B. bigemina are considered as the causative agents of bovine babesiosis; in addition, Babesia ovata and B. major are a group of benign bovine piroplasms. Therefore, species identification is important for diagnosis, epidemiological investigations and follow-up management.
methodsReal-time PCR combined with high resolution melting (RT-PCR-HRM) analysis was used to detect and discriminate four Babesia species infective to cattle, including Babesia bovis, B. bigemina, B. major and B. ovata. The melting profiles and melting temperatures (Tm) of the amplicon targeting 18S rRNA revealed differences that can discriminate the four Babesia spp. Sensitivity and specificity of the analytical method were evaluated using 50 blood samples collected from experimentally infected cattle and 240 blood samples from areas where bovine babesiosis is an issue.
resultsRT-PCR-HRM analysis allowed to detect and discriminate four Babesia spp. (B. bovis, B. bigemina, B. major and B. ovata), which were responsible for bovine babesiosis in China. The protocol was validated with DNA samples from experimentally infected cattle and field infection in cattle.
conclusionsOur results indicate that RT-PCR-HRM is a fast and robust tool for the simultaneous detection and discrimination of four Babesia species that are responsible for bovine babesiosis in China. This approach is applicable for both field and experimental samples, thus it could be useful in epidemiological investigations and diagnoses of bovine babesiosis.
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