ArticleBMC bioinformatics2019
Reconstruction and analysis of a Kluyveromyces marxianus genome-scale metabolic model.
Article in BMC bioinformatics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 60 citations in OpenAlex.
- HOM2 and TPK1 are associated with Kluyveromyces marxianus growth under high-temperature and reduced-aeration conditions.World journal of microbiology & biotechnology · 2026Article
- EngineeringFrontiers in microbiology · 2026Review
- Metabolic engineering and adaptive laboratory evolution of Kluyveromyces Marxianus for lactic acid production.Microbial cell factories · 2025Article
- Characterization and optimization of mnn11Δ-mediated enhancement in heterologous protein production in Kluyveromyces marxianus.Microbial cell factories · 2025Article
- From genetic constraints to process hurdles: A review ofCurrent research in food science · 2025Review
- Integration of proteomic data with genome-scale metabolic models: A methodological overview.Protein science : a publication of the Protein Society · 2024Review
- Article
- Snapshot of the Probiotic Potential ofFoods (Basel, Switzerland) · 2023Article
- Applications of genome-scale metabolic models to investigate microbial metabolic adaptations in response to genetic or environmental perturbations.Briefings in bioinformatics · 2023Review
- A Pan-Draft Metabolic Model Reflects Evolutionary Diversity across 332 Yeast Species.Biomolecules · 2022Article
- Reconstruction of a catalogue of genome-scale metabolic models with enzymatic constraints using GECKO 2.0.Nature communications · 2022Article
- merlin, an improved framework for the reconstruction of high-quality genome-scale metabolic models.Nucleic acids research · 2022Article
- Genome-scale modeling of yeast metabolism: retrospectives and perspectives.FEMS yeast research · 2022Review
- Unraveling the potential of non-conventional yeasts in biotechnology.FEMS yeast research · 2022Article
- Deletion ofFrontiers in microbiology · 2022Article
- Evaluating accessibility, usability and interoperability of genome-scale metabolic models for diverse yeasts species.FEMS yeast research · 2021Article
- Advances and Opportunities of CRISPR/Cas Technology in Bioengineering Non-conventional Yeasts.Frontiers in bioengineering and biotechnology · 2021Review
- Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions.Scientific reports · 2020Article
- The Model SystemLife (Basel, Switzerland) · 2020Review
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundKluyveromyces marxianus is a thermotolerant yeast with multiple biotechnological potentials for industrial applications, which can metabolize a broad range of carbon sources, including less conventional sugars like lactose, xylose, arabinose and inulin. These phenotypic traits are sustained even up to 45 °C, what makes it a relevant candidate for industrial biotechnology applications, such as ethanol production. It is therefore of much interest to get more insight into the metabolism of this yeast. Recent studies suggested, that thermotolerance is achieved by reducing the number of growth-determining proteins or suppressing oxidative phosphorylation. Here we aimed to find related factors contributing to the thermotolerance of K. marxianus.
resultsHere, we reported the first genome-scale metabolic model of Kluyveromyces marxianus, iSM996, using a publicly available Kluyveromyces lactis model as template. The model was manually curated and refined to include the missing species-specific metabolic capabilities. The iSM996 model includes 1913 reactions, associated with 996 genes and 1531 metabolites. It performed well to predict the carbon source utilization and growth rates under different growth conditions. Moreover, the model was coupled with transcriptomics data and used to perform simulations at various growth temperatures.
conclusionsK. marxianus iSM996 represents a well-annotated metabolic model of thermotolerant yeast, which provides a new insight into theoretical metabolic profiles at different temperatures of K. marxianus. This could accelerate the integrative analysis of multi-omics data, leading to model-driven strain design and improvement.
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