Evidence map›Paper›PMID 31694535›Full record

ArticleBMC genomics2019

Sex- and tissue-specific transcriptome analyses and expression profiling of olfactory-related genes in Ceracris nigricornis Walker (Orthoptera: Acrididae).

Hao Yuan, Huihui Chang, Lina Zhao, Chao Yang, Yuan Huang

Open access · goldAbstract read
In one paragraph

Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 19 citations in OpenAlex.

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  10. Innate Immune Responses ofFrontiers in cellular and infection microbiology · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Hao YuanCollege of Life Sciences, Shaanxi Normal University, Xi'an, 710062, China.
Huihui ChangCollege of Life Sciences, Shaanxi Normal University, Xi'an, 710062, China.
Lina ZhaoCollege of Life Sciences, Shaanxi Normal University, Xi'an, 710062, China.
Chao YangCollege of Life Sciences, Shaanxi Normal University, Xi'an, 710062, China.
Yuan HuangCollege of Life Sciences, Shaanxi Normal University, Xi'an, 710062, China. yuanh@snnu.edu.cn.ORCID http://orcid.org/0000-0001-7683-9193
Shaanxi Normal University · CNShaanxi Institute of Zoology · CN

Funding

National Natural Science Foundation of China 31872217; 31372192
6 · The paper itself

Abstract

backgroundThe sophisticated insect olfactory system plays an important role in recognizing external odors and enabling insects to adapt to environment. Foraging, host seeking, mating, ovipositing and other forms of chemical communication are based on olfaction, which requires the participation of multiple olfactory genes. The exclusive evolutionary trend of the olfactory system in Orthoptera insects is an excellent model for studying olfactory evolution, but limited olfaction research is available for these species. The olfactory-related genes of Ceracris nigricornis Walker (Orthoptera: Acrididae), a severe pest of bamboos, have not yet been reported.

resultsWe sequenced and analyzed the transcriptomes from different tissues of C. nigricornis and obtained 223.76 Gb clean data that were assembled into 43,603 unigenes with an N50 length of 2235 bp. Among the transcripts, 66.79% of unigenes were annotated. Based on annotation and tBLASTn results, 112 candidate olfactory-related genes were identified for the first time, including 20 odorant-binding proteins (OBPs), 10 chemosensory-binding proteins (CSPs), 71 odorant receptors (ORs), eight ionotropic receptors (IRs) and three sensory neuron membrane proteins (SNMPs). The fragments per kilobase per million mapped fragments (FPKM) values showed that most olfactory-related differentially expressed genes (DEGs) were enriched in the antennae, and these results were confirmed by detecting the expression of olfactory-related genes with quantitative real-time PCR (qRT-PCR). Among these antennae-enriched genes, some were sex-biased, indicating their different roles in the olfactory system of C. nigricornis.

conclusionsThis study provides the first comprehensive list and expression profiles of olfactory-related genes in C. nigricornis and a foundation for functional studies of these olfactory-related genes at the molecular level.

Indexed as

Gene Expression ProfilingSex CharacteristicsAnimalsGenes, InsectGrasshoppersMolecular Sequence AnnotationOrgan SpecificityRNA-SeqSequence Homology, Nucleic AcidSmellCeracris nigricornisChemosensory-binding proteinExpression profiles analysisIonotropic receptorOdorant-binding proteinOdorant receptorSensory neuron membrane proteinTranscriptome

Identifiers

PMID31694535
PMCPMC6836668
OpenAlexW2989299371

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.