ArticleBMC microbiology2019
Increase of reactive oxygen species contributes to growth inhibition by fluconazole in Cryptococcus neoformans.
Article in BMC microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- α-Terpineol reversesFrontiers in cellular and infection microbiology · 2026Article
- In vitro and in vivo efficacy of the antimycobacterial molecule SQ109 against the human pathogenic fungus, Cryptococcus neoformans.PLoS neglected tropical diseases · 2025Article
- Broad-Spectrum Activity and Mechanisms of Action of SQ109 on a Variety of Fungi.ACS infectious diseases · 2025Article
- Reconstruction and exploitation of a dedicated Genome-Scale Metabolic Model of the human pathogenbioRxiv : the preprint server for biology · 2025Article
- Unveiling new features of the human pathogenComputational and structural biotechnology journal · 2025Article
- Exposure of Cryptococcus neoformans to low nitrogen levels enhances virulence.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Article
- Functional Characterization of DNA N-Glycosylase Ogg1 and Ntg1 in DNA Damage Stress of Cryptococcus neoformans.Journal of microbiology (Seoul, Korea) · 2023Article
- Overcoming Drug Resistance in a ClinicalAntibiotics (Basel, Switzerland) · 2023Article
- Fungistatic Effect of Phthalide Lactones onMolecules (Basel, Switzerland) · 2023Article
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- Modulation of the nanoscale motion rate ofFrontiers in microbiology · 2023Article
- Reactive oxidant species induced by antifungal drugs: identity, origins, functions, and connection to stress-induced cell death.Frontiers in cellular and infection microbiology · 2023Review
- The Anticancer Drug Bleomycin Shows Potent Antifungal Activity by Altering Phospholipid Biosynthesis.Microbiology spectrum · 2022Article
- Lycosin-II Exhibits Antifungal Activity and Inhibits Dual-Species Biofilm byJournal of fungi (Basel, Switzerland) · 2022Article
- Synthesis of novel pyrroles and fused pyrroles as antifungal and antibacterial agents.Journal of enzyme inhibition and medicinal chemistry · 2021Article
- In vitro anti-Cryptococcus activity of diphenyl diselenide alone and in combination with amphotericin B and fluconazole.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Strain Degeneration inJournal of fungi (Basel, Switzerland) · 2021Article
- The Glycosylphosphatidylinositol-Anchored Superoxide Dismutase ofJournal of fungi (Basel, Switzerland) · 2021Article
- Effects of environmental factors on sensitivity of Cryptococcus neoformans to fluconazole and amphotericin B.FEMS microbiology letters · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCryptococcus neoformans, a basidiomycetous yeast, is a fungal pathogen that can colonize the lungs of humans causing pneumonia and fungal meningitis in severely immunocompromised individuals. Recent studies have implied that the antifungal drug fluconazole (FLC) can induce oxidative stress in C. neoformans by increasing the production of reactive oxygen species (ROS), as presence of the antioxidant ascorbic acid (AA) could reverse the inhibitory effects of FLC on C. neoformans. However, in Candida albicans, AA has been shown to stimulate the expression of genes essential for ergosterol biosynthesis. Hence, the contribution of ROS in FLC-mediated growth inhibition remains unclear.
resultsIn order to determine whether counteracting ROS generated by FLC in C. neoformans can contribute to diminishing inhibitory effects of FLC, we tested three other antioxidants in addition to AA, namely, pyrrolidine dithiocarbamate (PDTC), retinoic acid (RA), and glutathione (GSH). Our data confirm that there is an increase in ROS in the presence of FLC in C. neoformans. Importantly, all four antioxidants reversed FLC-mediated growth inhibition of C. neoformans to various extents. We further verified the involvement of increased ROS in FLC-mediated growth inhibition by determining that ROS-scavenging proteins, metallothioneins (CMT1 and CMT2), contribute to growth recovery by PDTC and AA during treatment with FLC.
conclusionOur study suggests that ROS contributes to FLC-mediated growth inhibition and points to a complex nature of antioxidant-mediated growth rescue in the presence of FLC.
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