Evidence map›Paper›PMID 31693273›Full record

ArticleJournal of mass spectrometry : JMS2020

Optimization of a minimal sample preparation protocol for imaging mass spectrometry of unsectioned juvenile invertebrates.

Katherine E Zink, Denise A Tarnowski, Mark J Mandel, Laura M Sanchez

Abstract read
In one paragraph

Article in Journal of mass spectrometry : JMS, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. ReducingAnalytical chemistry · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Katherine E ZinkDepartment of Pharmaceutical Sciences, University of Illinois at Chicago, 833 S Wood St, Chicago, IL, 60612, USA.
Denise A TarnowskiDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, 1550 Linden Dr, Madison, WI, 53706, USA.
Mark J MandelDepartment of Medical Microbiology and Immunology, University of Wisconsin-Madison, 1550 Linden Dr, Madison, WI, 53706, USA.
Laura M SanchezDepartment of Pharmaceutical Sciences, University of Illinois at Chicago, 833 S Wood St, Chicago, IL, 60612, USA.ORCID https://orcid.org/0000-0001-9223-7977

Funding

Genetic Analysis of Beneficial Bacterial ColonizationR35GM119627 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI MANDEL, MARK J · 2016 to 2021
$2.2M
Regulation of Bacterial Aggregation During Host ColonizationR21AI117262 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI MANDEL, MARK J · 2016 to 2017
$408k
Elucidating chemical cues in primary metastasisF31CA236237 · NCI · UNIVERSITY OF ILLINOIS AT CHICAGO · PI ZINK, KATHERINE · 2019 to 2019
$41k
Division of Integrative Organismal Systems 1757297National Institute of Allergy and Infectious Diseases R21AI117262NationalScience FoundationNCI NIH HHS F31 CA236237NCI NIH HHS F31CA236237NIAID NIH HHS R21 AI117262NIGMS NIH HHS R35 GM119627NIGMS NIH HHS R35GM119627NIH HHS
6 · The paper itself

Abstract

Tissue sections have long been the subject matter for the application of imaging mass spectrometry, but recently the technique has been adapted for many other purposes including bacterial colonies and 3D cell culture. Here, we present a simple preparation method for unsectioned invertebrate tissue without the need for fixing, embedding, or slicing. The protocol was used to successfully prepare a Hawaiian bobtail squid hatchling for analysis, and the resulting data detected ions that correspond to compounds present in the host only during its symbiotic colonization by Vibrio fischeri.

Indexed as

AgingAliivibrio fischeriAnimalsAnimal StructuresDecapodiformesDissectionInvertebratesSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSymbiosisEuprymna scolopesmicrobiologysymbiosisunsectioned organism imaging

Identifiers

PMID31693273
PMCPMC7145758

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.