Evidence map›Paper›PMID 31692683›Full record

ArticleBiotechnology reports (Amsterdam, Netherlands)2019

Covalently immobilized chemically modified lysozyme as a sorbent for bacterial endotoxins (lipopolysaccharides).

Pavel A Levashov, Darya A Matolygina, Oxana A Dmitrieva, Ekaterina D Ovchinnikova, Irina Yu Adamova, Nataliya V Karelina, Vladimir A Nelyub, Nikolay L Eremeev, Andrey V Levashov

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology reports (Amsterdam, Netherlands), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 22 citations in OpenAlex.

  1. Advances in the Development of Biomaterials for Endotoxin Adsorption in Sepsis.Frontiers in bioengineering and biotechnology · 2021
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Pavel A LevashovDepartment of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, Russia.
Darya A MatolyginaDepartment of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, Russia.
Oxana A DmitrievaInstitute of Experimental Cardiology, National Medical Research Center of Cardiology, Moscow, Russia.
Ekaterina D OvchinnikovaInstitute of Experimental Cardiology, National Medical Research Center of Cardiology, Moscow, Russia.
Irina Yu AdamovaInstitute of Experimental Cardiology, National Medical Research Center of Cardiology, Moscow, Russia.
Nataliya V KarelinaInterindustry Engineering Center for Composite Materials, N.E. Bauman Moscow State Technical University, Moscow, Russia.
Vladimir A NelyubInterindustry Engineering Center for Composite Materials, N.E. Bauman Moscow State Technical University, Moscow, Russia.
Nikolay L EremeevDepartment of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, Russia.
Andrey V LevashovDepartment of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, Russia.
Bauman Moscow State Technical University · RULomonosov Moscow State University · RUNational Medical Research Center of Cardiology · RUInstitute of Experimental Cardiology · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chemical modification of lysozyme was carried out using benzaldehyde and anisaldehyde. It was shown that chemical modification affects only 1-2 amino groups of the protein molecule which does not prevent further covalent immobilization of lysozyme using the remaining free amino groups. The bacteriolytic activity of lysozyme is preserved after chemical modification and after subsequent covalent immobilization. As a result of chemical modification immobilized lysozyme more effectively adsorbs bacterial lipopolysaccharides (endotoxins). Adsorption of immunoglobulin G does not increase after modification. The sorbents obtained in this work can be used for the future development of new medical material for the extracorporeal treatment of sepsis. The proposed scheme for the modification and immobilization of lysozyme can be used with various aldehydes for the preparation of sorbents with different properties.

Indexed as

AnisaldehydeBacteriolytic activityBenzaldehydeChemically modified lysozymeEndotoxin adsorptionImmobilized lysozymeImmunoglobulin G adsorptionSepsis

Identifiers

PMID31692683
PMCPMC6806385
OpenAlexW2977524300

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.