ArticleMicrobial biotechnology2020
The secretome of Pichia pastoris in fed-batch cultivations is largely independent of the carbon source but changes quantitatively over cultivation time.
Article in Microbial biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- Yarrowia lipolytica and Komagataella phaffii (Pichia pastoris) as Hosts for Heterologous Expression of Lipases.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Integrating dynamic modeling and continuous cultivation to enhance recombinant β-glucosidase production in Komagataella phaffii.Bioresources and bioprocessing · 2026Article
- Concentration and Purification of Proteins Produced by Pichia pastoris by TFF with Ceramic Membranes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Lytic Polysaccharide Monooxygenases as Reporters for Signal Peptide Evaluation in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Bulk and selective autophagy cooperate to remodel a fungal proteome in response to changing nutrient availability.Cell reports · 2025Article
- Metabolic engineering of yeast to efficiently synthesize heme and hemoproteins: recent advance and prospects.FEMS yeast research · 2025Review
- Recombinant production of Paenibacillus wynnii β-galactosidase with Komagataella phaffii.Microbial cell factories · 2024Article
- Biosynthesis of High-Active Hemoproteins by the Efficient Heme-Supply Pichia Pastoris Chassis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Emerging relevance of cell wall components from non-conventional yeasts as functional ingredients for the food and feed industry.Current research in food science · 2023Review
- Production, purification, and characterization of recombinant rabies virus glycoprotein expressed in PichiaPink™ yeast.Biotechnology reports (Amsterdam, Netherlands) · 2022Article
- Potential of the Signal Peptide Derived from theApplied and environmental microbiology · 2022Article
- Advances in Cell Engineering of theMetabolites · 2022Review
- Production of Single-Domain Antibodies in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022Article
- What makes Komagataella phaffii non-conventional?FEMS yeast research · 2021Review
- Identifying carbohydrate-active enzymes of Cutaneotrichosporon oleaginosus using systems biology.Microbial cell factories · 2021Article
- Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffii.Microbial cell factories · 2021Article
- Pseudohyphal differentiation in Komagataella phaffii: investigating the FLO gene family.FEMS yeast research · 2020Article
- The secretome of Pichia pastoris in fed-batch cultivations is largely independent of the carbon source but changes quantitatively over cultivation time.Microbial biotechnology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The quantitative changes of the secretome of recombinant Pichia pastoris (Komagataella phaffii) CBS7435 over the time-course of methanol- or glucose-limited fed-batch cultures were investigated by LC-ESI-MS/MS to define the carbon source-specific secretomes under controlled bioreactor conditions. In both set-ups, no indication for elevated cell lysis was found. The quantitative data revealed that intact and viable P. pastoris cells secrete only a low number of endogenous proteins (in total 51), even during high cell density cultivation. Interestingly, no marked differences in the functional composition of the P. pastoris secretome between methanol- and glucose-grown cultures were observed with only few proteins being specifically affected by the carbon source. The 'core secretome' of 22 proteins present in all analysed carbon sources (glycerol, glucose and methanol) consists mainly of cell wall proteins. The quantitative analysis additionally revealed that most secretome proteins were already present after the batch phase, and depletion rather than accumulation occurred during the fed-batch processes. Among the changes over cultivation time, the depletion of both the extracellularly detected chaperones and the only two identified proteases (Pep4 and Yps1-1) during the methanol- or glucose-feed phase appear as most prominent.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.