ArticleScientific reports2019
Nanopore sequencing improves the draft genome of the human pathogenic amoeba Naegleria fowleri.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 41 citations in OpenAlex.
- Adenine DNA methylation associated with transcriptionally permissive chromatin is widespread across eukaryotes.Nature genetics · 2025Article
- Structure-Based Insights into Stefin-Mediated Targeting of Fowlerpain-1: Towards Novel Therapeutics forPharmaceuticals (Basel, Switzerland) · 2025Article
- Comparative single-cell genomics of two uncultivatedmSphere · 2025Article
- Repressive Cytosine Methylation is a Marker of Viral Gene Transfer Across Divergent Eukaryotes.Molecular biology and evolution · 2025Article
- Characterization of co-infections of haemosporidian parasites in SwinhoeCurrent research in parasitology & vector-borne diseases · 2025Article
- Missing microbial eukaryotes and misleading meta-omic conclusions.Nature communications · 2024Article
- Exploration of the Binding Site of Arachidonic Acid in gp63 ofPathogens (Basel, Switzerland) · 2024Article
- Noninvasive diagnostic biomarkers, genomic profiling, and advanced microscopic imaging in the early detection and characterization ofAnnals of medicine and surgery (2012) · 2024Review
- In Silico Structural Analysis of Serine CarboxypeptidaseInternational journal of molecular sciences · 2022Article
- The Amazing Evolutionary Complexity of Eukaryotic Tubulins: Lessons fromFrontiers in cell and developmental biology · 2022Article
- Copper Metabolism inFrontiers in cell and developmental biology · 2022Article
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- High quality genome assembly of the amitochondriate eukaryoteMicrobial genomics · 2021Article
- Various brain-eating amoebae: the protozoa, the pathogenesis, and the disease.Frontiers of medicine · 2021Review
- The therapeutic potential of novel isobenzofuranones against Naegleria fowleri.International journal for parasitology. Drugs and drug resistance · 2021Article
- The transcriptome of Balamuthia mandrillaris trophozoites for structure-guided drug design.Scientific reports · 2021Article
- Naphthyridine Derivatives Induce Programmed Cell Death inPharmaceuticals (Basel, Switzerland) · 2021Article
- Comparative Genomic and Transcriptomic Analysis of Naegleria fowleri Clinical and Environmental Isolates.mSphere · 2021Article
- Genomics and transcriptomics yields a system-level view of the biology of the pathogen Naegleria fowleri.BMC biology · 2021Article
- Draft Chromosome Sequences of a Clinical Isolate of the Free-Living Ameba Naegleria fowleri.Microbiology resource announcements · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Naegleria fowleri is an environmental protist found in soil and warm freshwater sources worldwide and is known for its ability to infect humans and causing a rapid and mostly fatal primary amoebic meningoencephalitis. When contaminated water enters the nose, the facultative parasite follows the olfactory nerve and enters the brain by crossing the cribriform plate where it causes tissue damage and haemorrhagic necrosis. Although N. fowleri has been studied for several years, the mechanisms of pathogenicity are still poorly understood. Furthermore, there is a lack of knowledge on the genomic level and the current reference assembly is limited in contiguity. To improve the draft genome and to investigate pathogenicity factors, we sequenced the genome of N. fowleri using Oxford Nanopore Technology (ONT). Assembly and polishing of the long reads resulted in a high-quality draft genome whose N50 is 18 times higher than the previously published genome. The prediction of potentially secreted proteins revealed a large proportion of enzymes with a hydrolysing function, which could play an important role during the pathogenesis and account for the destructive nature of primary amoebic meningoencephalitis. The improved genome provides the basis for further investigation unravelling the biology and the pathogenic potential of N. fowleri.
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